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Rabbit Recombinant Monoclonal SS18 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (AB254405), expandable thumbnail
  • Western blot - Anti-SS18 antibody [EPR23636-98] (AB254405), expandable thumbnail
  • Western blot - Anti-SS18 antibody [EPR23636-98] (AB254405), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (AB254405), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (AB254405), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Expected
Not recommended
Tested
Tested
Rat
Not recommended
Expected
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:29374058).

Alternative names

SSXT, SYT, SS18, Protein SSXT, Protein SYT, Synovial sarcoma translocated to X chromosome protein

Recommended products

Rabbit Recombinant Monoclonal SS18 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23636-98
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (ab254405)

    SS18 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab254405 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254405 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab254405 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254405 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (ab254405)

    Predicted band size: 46 kDa

    Observed band size: 45 kDa, 55 kDa

  • Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405)

    False colour image of Western blot: Anti-SS18 antibody [EPR23636-98] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab254405 was shown to bind specifically to SS18. A band was observed at 51/56 kDa in wild-type HeLa cell lysates with no signal observed at this size in SS18 knockout cell line Human SS18 knockout HeLa cell line ab265493 (knockout cell lysate Human SS18 knockout HeLa cell lysate ab259156). To generate this image, wild-type and SS18 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: SS18 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human SS18 knockout HeLa cell line (Human SS18 knockout HeLa cell line ab265493)

    Lane 3: HEK-293 cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 46 kDa

    Observed band size: 51 kDa, 56 kDa

  • Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:23540691;29374058)

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure times: Lanes 1-3: 59 seconds; Lane 4: 3 minutes; Lane 5: 59 seconds.

    All lanes: Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa

    Observed band size: 45 kDa, 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human cervical carcinoma. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

    Immunohistochemical analysis of paraffin-embedded Human synovial sarcoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in Human synovial sarcoma (PMID: 27120803). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse colon. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat cardiac muscle. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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