Anti-SS18-SSX Fusion Protein antibody [EPR24159-81]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling SS18-SSX Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control : No staining on human kidney.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged SSX18-SSX2 fusion construct (right panel) stained for SS18-SSX Fusion Protein using ab254406 at a 1/5000 dilution (0.01 μg), followed by Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

Isotype control (left panel) is Rabbit monoclonal IgG (ab172730).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SSXT-SSX2 labeled 6X His tag and (Panel B) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. labeling SS18-SSX Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Nuclear staining on (A) HEK-293T transfected with SSXT-SSX2 labeled 6X His tag, no staining on (B) HEK-293T transfected with an empty plasmid.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling SS18-SSX Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control : No staining on human stomach.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue labeling SS18-SSX Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Nuclear staining on human synovial sarcoma (PMID : 32141887).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc tagged non-fused SS18+SSX1+SSX2+SSX4 constructs (right panel) stained for SS18-SSX Fusion Protein using ab254406 at a 1/5000 dilution (0.01 μg), followed by Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at a 1/2000 dilution.

Isotype control (left panel) is Rabbit monoclonal IgG (ab172730).

• Dot

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Dot Blot - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (AB254406)

Dot Blot - Anti-SS18-SSX Fusion Protein antibody [EPR24159-81] (ab254406 used at a 1/1000 dilution (0.592 μg/ml)).

Lane 1 : Human SS18 + SSX(1/2/4) fusion peptide

Lane 2 : Human SS18 peptide

Lane 3 : Human SSX(1/2/4) peptide

Blocking/Dilution buffer : 5% NFDM/TBST.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.

Exposure time is 3 mins.