AB124932

Anti-SSB antibody [EPR6570]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal SSB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-B, SSB

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (AB124932)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (AB124932)

Formalin-fixed, paraffin-embedded human thyroid adenocarcinoma tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (AB124932)

Formalin-fixed, paraffin-embedded human colon tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SSB antibody [EPR6570] (AB124932)

All lanes:

Western blot - Anti-SSB antibody [EPR6570] (ab124932) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Ramos cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Lane 7:

NIH 3T3 cell lysate at 10 µg

Lane 8:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 47 kDa

false

OI-RD Scanning - Anti-SSB antibody [EPR6570] (AB124932)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SSB antibody [EPR6570] (AB124932)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-SSB antibody [EPR6570] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6570

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The single-stranded DNA-binding protein (SSB) also known as La protein in humans has a mechanical role in stabilizing single-stranded DNA (ssDNA) during replication recombination and repair processes. In E. coli the SSB protein weighs approximately 19 kDa and is essential for maintaining genome integrity. SSB expression occurs in various organisms with bacterial SSBs being key to prokaryotic cells while homologs like La/SSB in humans are more involved in post-transcriptional processes. Anti-SSB antibodies target this protein often in research and diagnostic applications.

Biological function summary

The single-stranded DNA-binding protein plays an important role in the orchestration of DNA metabolism. It binds to ssDNA substrates acting as a scaffold for recruiting other proteins and it frequently involves itself in complex formation with other cellular factors such as replication proteins. This function proves important in stabilizing unwound DNA and regulating access for proteins involved in DNA synthesis and repair. Anti-La SSB antibodies are typically used to study aberrant RNA processing events.

Pathways

The SSB protein influences the DNA replication and repair pathways. SSB's interaction with proteins like DNA polymerase and exonuclease suggests its cooperation with these enzymes to ensure precise DNA synthesis and to facilitate proper repair of DNA. Anti-SSB Ab can be used to investigate specific pathway dysregulations providing insights into pathway alterations that impact genome stability. Notably SSB's role in coordinating with other repair proteins emphasizes its centrality in maintaining steady state of DNA integrity.

The single-stranded DNA-binding protein has vital connections to autoimmune diseases. Sjögren's syndrome and systemic lupus erythematosus often see the presence of anti-SSB antibodies indicating an autoimmune response against this protein. These disorders associate with the human La protein an autoimmune target in these disease states. Understanding SSB's interaction with other proteins such as Ro60 in these conditions gives insights into disease pathology and potential areas for therapeutic intervention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed : 2470590, PubMed : 3192525). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed : 12384597).

See full target information Lupus La protein

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e38702 PubMed39641036

2024

SSB expression is associated with metabolic parameters of F-FDG PET/CT in lung adenocarcinoma and can improve diagnostic efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Yue Liu,Ling-Ling Yuan,Yan Gao,Yu Zhang,Yao-Hua Zhang,Yi Yang,Yu-Xuan Chen,Xu-Sheng Liu,Zhi-Jun Pei

Autophagy 20:1314-1334 PubMed38174993

2024

Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhen Zhang,Mengyi Lao,Hanshen Yang,Kang Sun,Yunfei Dong,Lihong He,Xinchi Jiang,Honghui Wu,Yangwei Jiang,Muchun Li,Honggang Ying,Xinyuan Liu,Jian Xu,Yan Chen,Hanjia Zhang,Ruhong Zhou,Jianqing Gao,Xueli Bai,Tingbo Liang

Frontiers in oncology 12:763480 PubMed36110943

2022

La protein regulates protein expression by binding with the mRNAs of target genes and participates the pathological process of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Huang,Jialei Zhu,Yueyan Li,Yang Yu,Jing Tang

Asia-Pacific journal of clinical oncology 16:e228-e235 PubMed32780941

2020

La protein contributes to cells proliferation and migration and serves as a potential therapeutic target for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqian Pan,Shuangmei Tong,Lingjun Kong,Jialei Zhu,Jing Tang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSB antibody [EPR6570]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (AB124932)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (AB124932)

Western blot - Anti-SSB antibody [EPR6570] (AB124932)

OI-RD Scanning - Anti-SSB antibody [EPR6570] (AB124932)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise