Rabbit Recombinant Monoclonal SSB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] - BSA and Azide free (AB232025), expandable thumbnail](https://content.abcam.com/products/images/ssb-antibody-epr6570-bsa-and-azide-free-ab232025--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125675.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] - BSA and Azide free (AB232025)")
![OI-RD Scanning - Anti-SSB antibody [EPR6570] - BSA and Azide free (AB232025), expandable thumbnail](https://content.abcam.com/products/images/ssb-antibody-epr6570-bsa-and-azide-free-ab232025--oi-rd-scanning-img95300.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-SSB antibody [EPR6570] - BSA and Azide free (AB232025)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Expected |
| Rat | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed:3192525, PubMed:2470590). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12384597).
Alternative names
Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-B, SSB
Recommended products
Rabbit Recombinant Monoclonal SSB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Alternative names
Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-B, SSB
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR6570
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232025 is the carrier-free version of Anti-SSB antibody [EPR6570] ab124932.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The single-stranded DNA-binding protein (SSB) also known as La protein in humans has a mechanical role in stabilizing single-stranded DNA (ssDNA) during replication recombination and repair processes. In E. coli the SSB protein weighs approximately 19 kDa and is essential for maintaining genome integrity. SSB expression occurs in various organisms with bacterial SSBs being key to prokaryotic cells while homologs like La/SSB in humans are more involved in post-transcriptional processes. Anti-SSB antibodies target this protein often in research and diagnostic applications.
- Biological function summary
The single-stranded DNA-binding protein plays an important role in the orchestration of DNA metabolism. It binds to ssDNA substrates acting as a scaffold for recruiting other proteins and it frequently involves itself in complex formation with other cellular factors such as replication proteins. This function proves important in stabilizing unwound DNA and regulating access for proteins involved in DNA synthesis and repair. Anti-La SSB antibodies are typically used to study aberrant RNA processing events.
- Pathways
The SSB protein influences the DNA replication and repair pathways. SSB's interaction with proteins like DNA polymerase and exonuclease suggests its cooperation with these enzymes to ensure precise DNA synthesis and to facilitate proper repair of DNA. Anti-SSB Ab can be used to investigate specific pathway dysregulations providing insights into pathway alterations that impact genome stability. Notably SSB's role in coordinating with other repair proteins emphasizes its centrality in maintaining steady state of DNA integrity.
- Associated diseases and disorders
The single-stranded DNA-binding protein has vital connections to autoimmune diseases. Sjögren's syndrome and systemic lupus erythematosus often see the presence of anti-SSB antibodies indicating an autoimmune response against this protein. These disorders associate with the human La protein an autoimmune target in these disease states. Understanding SSB's interaction with other proteins such as Ro60 in these conditions gives insights into disease pathology and potential areas for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] - BSA and Azide free (ab232025), expandable thumbnail](https://content.abcam.com/products/images/ssb-antibody-epr6570-bsa-and-azide-free-ab232025--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125675.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] - BSA and Azide free (ab232025)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] - BSA and Azide free (ab232025)
Formalin-fixed, paraffin-embedded human thyroid adenocarcinoma tissue stained for SSB with Anti-SSB antibody [EPR6570] ab124932 (1/250 dilution) in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SSB antibody [EPR6570] ab124932).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-SSB antibody [EPR6570] - BSA and Azide free (ab232025), expandable thumbnail](https://content.abcam.com/products/images/ssb-antibody-epr6570-bsa-and-azide-free-ab232025--oi-rd-scanning-img95300.jpg "OI-RD Scanning - Anti-SSB antibody [EPR6570] - BSA and Azide free (ab232025)")
OI-RD Scanning - Anti-SSB antibody [EPR6570] - BSA and Azide free (ab232025)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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