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Rabbit Polyclonal SSBP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SSBP1 aa 1 to C-terminus.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SSBP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q04837
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Orangutan
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Orangutan

Dilution info

-

Notes

-

Target data

Function

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed:21953457, PubMed:23290262). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the replisome components POLG and TWNK at the replication fork (PubMed:21953457, PubMed:12975372, PubMed:26446790, PubMed:15167897). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed:26446790, PubMed:21953457). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed:12975372). May also function in mtDNA repair (PubMed:23290262).

Alternative names

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Rabbit Polyclonal SSBP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SSBP1 aa 1 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SSBP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q04837
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Single-Stranded DNA Binding Protein 1 participates in essential mitochondrial DNA maintenance processes and interacts with other proteins to form functional complexes. It associates with mitochondrial DNA polymerase gamma and other replication machinery components to ensure accurate DNA replication. These interactions are significant for proper mitochondrial function since they prevent mutations that could compromise cellular energy production.

Activity summary

SSBP1 also known as Single-Stranded DNA Binding Protein 1 is a mitochondrial protein with an approximate mass of 14 kDa. SSBP1 binds to single-stranded DNA and stabilizes it during DNA replication and repair processes. It functions by preventing the DNA from re-annealing or forming secondary structures which helps in maintaining genomic stability. This protein is mostly expressed in tissues with high energy demands such as muscle and liver reflecting its significant role in mitochondrial activities.

Pathways

SSBP1 engages actively in the mitochondrial DNA replication and repair pathway. It works closely with proteins like TWINKLE helicase and DNA polymerase gamma complex facilitating efficient replication and repair of mitochondrial DNA. These pathways are pivotal for mitochondrial biogenesis and function impacting cellular energy metabolism and overall mitochondrial health.

Associated diseases and disorders

Alterations in SSBP1 have links to mitochondrial dysfunctions including disorders like mitochondrial myopathy and progressive external ophthalmoplegia. Dysfunctional SSBP1 may lead to impaired DNA replication or repair in mitochondria contributing to these conditions. Additionally it relates to TWINKLE helicase mutations which also disrupt mitochondrial DNA maintenance further emphasizing the importance of SSBP1 in maintaining cellular energy balance.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human testis sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human cerebral cortex sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human duodenum sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab224053)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labeling SSBP1 with ab224053 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (ab224053), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (ab224053)

    PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SSBP1 (green) using ab224053 at 4 μg/ml in ICC/IF.

  • Western blot - Anti-SSBP1 antibody (ab224053), expandable thumbnail

    Western blot - Anti-SSBP1 antibody (ab224053)

    All lanes: Western blot - Anti-SSBP1 antibody (AB224053) at 1/100 dilution

    Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lane 2: NBT-II cell lysate

    Predicted band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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