AB74710

Anti-SSBP1 antibody

Name: Anti-SSBP1 antibody (ab74710) | Abcam
Brand: Abcam Limited
SKU: AB74710
Price: 545 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal SSBP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SSBP1.

View Alternative Names

SSBP, SSBP1, Mt-SSB, MtSSB, PWP1-interacting protein 17

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB74710)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB74710)

Immunofluorescence analysis of A549 cells, using ab74710 at a dilution of 1/500. Left-hand panel : no peptide; right-hand panel : with peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB74710)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB74710)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ab74710 at a dilution of 1/50. Left-hand panel : no peptide; right-hand panel : with peptide.

Unknown

Western blot - Anti-SSBP1 antibody (AB74710)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-SSBP1 antibody (ab74710) at 1/500 dilution

Lane 1:

Extracts from HeLa cells at 5 µg with no peptide

Lane 2:

Extracts from HUVEC cells at 5 µg with no peptide

Lane 3:

Extracts from HUVEC cells at 5 µg with peptide

Predicted band size: 17 kDa

Observed band size: 17 kDa,26 kDa,45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SSBP1. The exact immunogen used to generate this antibody is proprietary information.

Q04837

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSBP1 also known as Single-Stranded DNA Binding Protein 1 is a mitochondrial protein with an approximate mass of 14 kDa. SSBP1 binds to single-stranded DNA and stabilizes it during DNA replication and repair processes. It functions by preventing the DNA from re-annealing or forming secondary structures which helps in maintaining genomic stability. This protein is mostly expressed in tissues with high energy demands such as muscle and liver reflecting its significant role in mitochondrial activities.

Biological function summary

Single-Stranded DNA Binding Protein 1 participates in essential mitochondrial DNA maintenance processes and interacts with other proteins to form functional complexes. It associates with mitochondrial DNA polymerase gamma and other replication machinery components to ensure accurate DNA replication. These interactions are significant for proper mitochondrial function since they prevent mutations that could compromise cellular energy production.

Pathways

SSBP1 engages actively in the mitochondrial DNA replication and repair pathway. It works closely with proteins like TWINKLE helicase and DNA polymerase gamma complex facilitating efficient replication and repair of mitochondrial DNA. These pathways are pivotal for mitochondrial biogenesis and function impacting cellular energy metabolism and overall mitochondrial health.

Alterations in SSBP1 have links to mitochondrial dysfunctions including disorders like mitochondrial myopathy and progressive external ophthalmoplegia. Dysfunctional SSBP1 may lead to impaired DNA replication or repair in mitochondria contributing to these conditions. Additionally it relates to TWINKLE helicase mutations which also disrupt mitochondrial DNA maintenance further emphasizing the importance of SSBP1 in maintaining cellular energy balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed : 21953457, PubMed : 23290262, PubMed : 31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed : 12975372, PubMed : 15167897, PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed : 21953457, PubMed : 26446790, PubMed : 31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed : 12975372). May also function in mtDNA repair (PubMed : 23290262).

See full target information SSBP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 10:1393 PubMed32082360

2020

RNase H1 Regulates Mitochondrial Transcription and Translation the Degradation of 7S RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Joanna Rusecka,Katarzyna Tońska,Massimo Zeviani

Oncotarget 8:110861-110876 PubMed29340022

2017

A functional polymorphism of gene predicts prognosis and response to chemotherapy in resected gastric cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuchen Li,Falin Qu,Renli Li,Xianli He,Yulong Zhai,Weigang Chen,Yong Zheng

DNA repair 12:177-87 PubMed23290262

2013

Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB).

Applications

Unspecified application

Species

Unspecified reactive species

Barbara van Loon,Leona D Samson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (AB74710)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB74710)

Western blot - Anti-SSBP1 antibody (AB74710)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise