AB177944

Anti-SSBP2 antibody [EPR11520]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal SSBP2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

View Alternative Names

SSDP2, SSBP2, Single-stranded DNA-binding protein 2, Sequence-specific single-stranded-DNA-binding protein 2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (AB177944)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma tissue labeling SSBP2 with ab177944 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SSBP2 with ab177944 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SSBP2 with ab177944 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SSBP2 antibody [EPR11520] (AB177944)

All lanes:

Western blot - Anti-SSBP2 antibody [EPR11520] (ab177944) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

Fetal heart tissue lysate at 10 µg

Predicted band size: 38 kDa

false

Carrier free

Anti-SSBP2 antibody [EPR11520] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11520

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Single-stranded DNA-binding protein 2 (SSBP2) also known as hSSB2 is a protein with a molecular mass of approximately 38 kDa. This protein plays a role in binding to single-stranded DNA. It is highly expressed in hematopoietic tissues including bone marrow and blood cells. SSBP2 also shows distinct expression in multiple human tissues highlighting its importance across different cellular environments. Mechanistically SSBP2 stabilizes single-stranded DNA by preventing the formation of secondary structures which is critical during DNA replication and repair.

Biological function summary

SSBP2 functions as a part of the multi-protein complex important for DNA metabolism and maintenance of genomic stability. It plays a role in the transcriptional activation of several tumor suppressor genes by interacting with other single-stranded DNA-binding partners. The protein influences cell cycle regulation by modulating the expression of genes involved in cell division and proliferation. This biological action affirms SSBP2's contribution to maintaining normal cellular functions and preventing abnormal cellular growth.

Pathways

The involvement of SSBP2 in cell cycle regulation and DNA repair underlines its participation in pathways like p53 signaling and homologous recombination. SSBP2 connects with proteins like RPA which is central to DNA metabolism to ensure accurate DNA replication and repair. These interactions place SSBP2 in a critical position to respond to and repair DNA damage thereby maintaining cellular integrity and genomic stability.

SSBP2 has relevance in leukemia and breast cancer. Its reduced expression or inactivity correlates with the progression of these diseases. In leukemia SSBP2's interaction with tumor suppressor proteins such as pRB is significant. In breast cancer its dysregulation can lead to unchecked cell proliferation. Understanding these relationships provides insight into SSBP2's potential as a biomarker and therapeutic target in cancer research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SSBP2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:322 PubMed41024119

2025

Transcription factor MYB regulates glycolysis to promote the transfer and malignant progression of gastric cancer through the SSBP2/ISL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenni Sun,Mi Zhou,Yasai Yao,Xuehong Chen,Tao Qin,Yafei Han,Lu Yue,Ruyong Yao

Biomedicines 11: PubMed37509458

2023

Immunohistochemical Analysis of Single-Stranded DNA Binding Protein 2 in Non-Melanoma Skin Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Seongsik Bang,Hwangkyu Son,Hyebin Cha,Kihyuk Song,Hosub Park,Hyunsung Kim,Joo Yeon Ko,Jaekyung Myung,Seungsam Paik

Diagnostics (Basel, Switzerland) 12: PubMed35204577

2022

Loss of Single-Stranded DNA Binding Protein 2 Expression Is Associated with Aggressiveness and Poor Overall Survival in Patients with Invasive Breast Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hosub Park,Seungyun Jee,Hwangkyu Son,Hyebin Cha,Seongsik Bang,Hyunsung Kim,Su-Jin Shin,Chihwan Cha,Min Sung Chung,Jaekyung Myung,Seung Sam Paik

Diagnostics (Basel, Switzerland) 10: PubMed33339271

2020

Nuclear Expression Loss of SSBP2 Is Associated with Poor Prognostic Factors in Colorectal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yumin Chung,Hyunsung Kim,Seongsik Bang,Kiseok Jang,Seung Sam Paik,Su-Jin Shin

PloS one 15:e0236896 PubMed32745119

2020

The loss of nuclear expression of single-stranded DNA binding protein 2 of gastric adenocarcinoma and its prognostic role: Analysis of molecular subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Seongsik Bang,Hyunsung Kim,Kiseok Jang,Seung Sam Paik,Su-Jin Shin

PloS one 15:e0232585 PubMed32374750

2020

Comparison of a human neuronal model proteome upon Japanese encephalitis or West Nile Virus infection and potential role of mosquito saliva in neuropathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Benoit Besson,Justine Basset,Sandrine Gatellier,Hélène Chabrolles,Thibault Chaze,Véronique Hourdel,Mariette Matondo,Nathalie Pardigon,Valérie Choumet

In vivo (Athens, Greece) 34:101-107 PubMed31882468

2019

Low Expression of Single-stranded DNA Binding Protein 2 (SSBP2) Predicts Unfavourable Postoperative Outcomes in Patients With Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsung Kim,Yeseul Kim,Seongsik Bang,Seongeon Park,Seungyun Jee,Jongmin Sim,Su-Jin Shin,Seung Sam Paik,Kiseok Jang

BMC cancer 18:1244 PubMed30541499

2018

Single-stranded DNA binding protein 2 expression is associated with patient survival in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsung Kim,Yeseul Kim,Yumin Chung,Rehman Abdul,Jongmin Sim,Hyein Ahn,Su-Jin Shin,Seung Sam Paik,Han Joon Kim,Kiseok Jang,Dongho Choi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSBP2 antibody [EPR11520]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Flow Cytometry (Intracellular) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (AB177944)

Western blot - Anti-SSBP2 antibody [EPR11520] (AB177944)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise