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Mouse Monoclonal FUT4 antibody. Suitable for IHC-FoFr, IHC-P, ICC, Flow Cyt and reacts with Human, Mouse samples. Cited in 62 publications.


Images

Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (AB16285), expandable thumbnail
  • Flow Cytometry - Anti-SSEA1 antibody [MC-480] (AB16285), expandable thumbnail
  • Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (AB16285), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (AB16285), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 99% Tissue culture supernatant

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FoFrIHC-PICCFlow Cyt
Human
Expected
Tested
Expected
Expected
Mouse
Predicted
Expected
Tested
Tested

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Fixation with 100% MeOH (5 min) or 4% PFA (10 min).

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed:11485743). Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed:11485743). Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (PubMed:11485743). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (By similarity).

Alternative names

Elft, Fut4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Galactoside 3-L-fucosyltransferase, Fuc-TIV, FucT-IV

Recommended products

Mouse Monoclonal FUT4 antibody. Suitable for IHC-FoFr, IHC-P, ICC, Flow Cyt and reacts with Human, Mouse samples. Cited in 62 publications.

Key facts

Isotype
IgM
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MC-480
Purity
Tissue culture supernatant
Specificity

We guarantee IHC-P in Human only

Light chain type
kappa
Concentration
Loading...
Purification notes

Tissue culture supernatant was cross flow concentrated.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Stage-specific embryonic antigen-1 (SSEA-1) also known as CD15 is a carbohydrate antigen often attached to glycoproteins or glycolipids. It has a molecular mass of approximately 480 Da. SSEA-1 is prominently expressed on the surface of murine embryonal carcinoma cells granulocytes and neural stem cells. It plays a role in cellular processes such as adhesion where it influences cell-to-cell and cell-to-extracellular matrix interactions.

Biological function summary

SSEA-1 participates in the regulation of cell differentiation and migration. This carbohydrate antigen is particularly critical in developmental stages where it impacts cellular behaviors that are important for embryogenesis. It functions as an adhesion molecule playing a role in forming complexes with cell surface lectins. These complexes can influence how cells communicate and adhere to one another therefore affecting various aspects of cellular dynamics.

Pathways

SSEA-1 is integral to the leukocyte extravasation signaling pathway which is significant for the immune system's operation. It interacts with proteins involved in the regulation of inflammation and immune response such as selectins. The antigen aids the tethering and rolling of leukocytes on endothelial surfaces a precursor step for their transmigration into tissues. SSEA-1's interaction with selectins is important in this process driving important cellular functions that enable immune surveillance and responses.

Associated diseases and disorders

SSEA-1 is associated with certain cancers such as leukemia and neurological disorders. In leukemia SSEA-1 can act as a marker for cancer stem cells influencing tumor progression and resistance to chemotherapy. In the context of neurological disorders its altered expression is connected with the pathology of Alzheimer's disease where neural stem cell behavior may be affected. Other proteins like amyloid precursors potentially interact with SSEA-1 in Alzheimer's contributing to the disease's complexity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285), expandable thumbnail

    Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)

    ab16285 staining SSEA1 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16285 at a 1/1000 dilution and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285), expandable thumbnail
    This image is taken from an anonymous customer review.

    Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285)

    Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

  • Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285), expandable thumbnail
    This image is courtesy of the laboratory of Peter Andrews.

    Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)

    The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)

    IHC image of SSEA1 staining in Human Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1/200 dilution, overnight at 4°C. A Goat anti-Mouse (IgM) HRP conjugated secondary antibody (Goat Anti-Mouse IgM mu chain (HRP) ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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