Anti-SSEA1 antibody [MC-480]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (AB16285)

IHC image of SSEA1 staining in Human Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1/200 dilution, overnight at 4°C. A Goat anti-Mouse (IgM) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ICC

Lab

Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (AB16285)

ab16285 staining SSEA1 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16285 at a 1/1000 dilution and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor^® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• ICC

Project752****

Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (AB16285)

The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.

This image is courtesy of the laboratory of Peter Andrews.

• Flow Cyt

Collaborator

Flow Cytometry - Anti-SSEA1 antibody [MC-480] (AB16285)

Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1 : 100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

This image is taken from an anonymous abreview.