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AB16286

Anti-SSEA3 antibody [MC631]

4

(2 Reviews)

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(40 Publications)

Rat Monoclonal SSEA3 antibody. Suitable for IHC-FoFr, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 40 publications.

View Alternative Names

Stage specific embryonic antigen 3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (AB16286)
  • ICC/IF

Project

Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (AB16286)

The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286.

This image is courtesy of the laboratory of Peter Andrews.

Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286)
  • Flow Cyt

Characteriser

Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286)

Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.

This image is courtesy of Prof Chris Denning's lab, University of Nottingham

Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286)
  • Flow Cyt

Collaborator

Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286)

Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).

This image is from an anonymous collaborator.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MC631

Isotype

IgM

Carrier free

No

Reacts with

Human

Applications

IHC-FoFr, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igm-kappa-monoclonal-rtk2118-isotype-control-ab35768'>ab35768</a> - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

The monoclonal antibody that defines SSEA-3 was derived by fusing cells of the murine myeloma cell line SP2/0 with the spleen cells of a Fisher rat immunized with 4-8-cell-stage zonapellucida-free embryos from randomly bred mice.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Tissue culture supernatant was cross flow concentrated.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSEA3 also known as Stage-Specific Embryonic Antigen-3 is a glycosphingolipid with a molecular mass not specifically defined due to its lipid nature. This antigen is expressed on the surface of pluripotent stem cells particularly human embryonic stem cells and some germ cells. SSEA3 belongs to the globoseries of glycolipids and is widely used as a marker for undifferentiated cells. The expression of SSEA3 decreases as these cells undergo differentiation which makes it useful in studying stem cell biology and early development processes.
Biological function summary

SSEA3 plays a role in cell adhesion and signaling during early embryogenesis. It does not form a complex itself but exists in the cell membrane contributing to interactions with other molecules or possibly engaging in signaling processes essential for cell proliferation and maintenance of stemness. The role of SSEA3 as a marker in detecting undifferentiated cells uncovers its importance in maintaining specific cell states.

Pathways

SSEA3 may influence cellular pathways that govern stem cell maintenance and differentiation. Though not directly a pathway component it associates with proteins involved in pluripotency regulation like the Wnt and Notch pathways which are important for determining cell fate and growth. These pathways involve connections to proteins such as beta-catenin in the Wnt pathway which can influence stemness and cellular development.

Alterations in SSEA3 expression have been related to certain cancers and stem cell-related diseases. SSEA3 is sometimes found in higher levels in cancers that exhibit stem cell-like characteristics such as certain teratocarcinomas. It connects to proteins implicated in oncogenesis through shared expression patterns in tumors and stem cells like the Oct4 protein which is known for its role in maintaining stem cell properties and is often reactivated in cancer cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SSEA3
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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 29:e13528 PubMed38009041

2023

Characteristics of multilineage-differentiating stress-enduring cell clusters in different culture conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ning,Yan-Yun Cao,Ru-Zhi Zhang,Yue Li

Scientific reports 12:17507 PubMed36266301

2022

High throughput screening of mesenchymal stem cell lines using deep learning.

Applications

Unspecified application

Species

Unspecified reactive species

Gyuwon Kim,Jung Ho Jeon,Keonhyeok Park,Sung Won Kim,Do Hyun Kim,Seungchul Lee

Development, growth & differentiation 64:325-341 PubMed35841539

2022

Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Yoshimatsu,Atsushi Yamazaki,Kazuya Edamura,Yuko Koushige,Hisashi Shibuya,Emi Qian,Tsukika Sato,Junko Okahara,Noriyuki Kishi,Toshiaki Noce,Yoshifumi Yamaguchi,Hideyuki Okano

EMBO reports 23:e54027 PubMed35289477

2022

Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Ammar Jabali,Anne Hoffrichter,Ana Uzquiano,Fabio Marsoner,Ruven Wilkens,Marco Siekmann,Bettina Bohl,Andrea C Rossetti,Sandra Horschitz,Philipp Koch,Fiona Francis,Julia Ladewig

Biomedicines 9: PubMed34829971

2021

Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Alena Kashirina,Alena Gavrina,Emil Kryukov,Vadim Elagin,Yuliya Kolesova,Alexander Artyuhov,Ekaterina Momotyuk,Vepa Abdyyev,Natalia Meshcheryakova,Elena Zagaynova,Erdem Dashinimaev,Aleksandra Kashina

Stem cell research & therapy 12:402 PubMed34256823

2021

Potential application of human neural crest-derived nasal turbinate stem cells for the treatment of neuropathology and impaired cognition in models of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yeon Lim,Sang In Park,Soon A Park,Jung Ho Jeon,Ho Yong Jung,Jung-Min Yon,Sin-Soo Jeun,Hyun Kook Lim,Sung Won Kim

Cell stem cell 28:1549-1565.e12 PubMed33915080

2021

Principles of signaling pathway modulation for enhancing human naive pluripotency induction.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Bayerl,Muneef Ayyash,Tom Shani,Yair Shlomo Manor,Ohad Gafni,Rada Massarwa,Yael Kalma,Alejandro Aguilera-Castrejon,Mirie Zerbib,Hadar Amir,Daoud Sheban,Shay Geula,Nofar Mor,Leehee Weinberger,Segev Naveh Tassa,Vladislav Krupalnik,Bernardo Oldak,Nir Livnat,Shadi Tarazi,Shadi Tawil,Emilie Wildschutz,Shahd Ashouokhi,Lior Lasman,Varda Rotter,Suhair Hanna,Dalit Ben-Yosef,Noa Novershtern,Sergey Viukov,Jacob H Hanna

Stem cells (Dayton, Ohio) 39:866-881 PubMed33621399

2021

A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair, generating improved genetically stable human induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Belén Alvarez-Palomo,Jordi Requena-Osete,Raul Delgado-Morales,Victoria Moreno-Manzano,Carme Grau-Bove,Agueda M Tejera,Manel Juan Otero,Carme Barrot,Irene Santos-Barriopedro,Alejandro Vaquero,Jovita Mezquita-Pla,Sebastian Moran,Carlos Hobeich Naya,Iris Garcia-Martínez,Francisco Vidal Pérez,María A Blasco,Manel Esteller,Michael J Edel

Laryngoscope investigative otolaryngology 5:903-910 PubMed33134538

2020

Heterogeneity and hierarchy of the tissue stem cells in the human newborn vocal fold mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Kiminori Sato,Shun-Ichi Chitose,Fumihiko Sato,Kiminobu Sato,Takeharu Ono,Hirohito Umeno

Acta histochemica 121:164-170 PubMed30558911

2018

Differentiation potential of the cells in the macula flava of the human vocal fold mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Fumihiko Sato,Shun-Ichi Chitose,Kiminori Sato,Takashi Kurita,Kiminobu Sato,Hirohito Umeno,Hirohisa Yano
View all publications
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