Rat Monoclonal SSEA3 antibody. Suitable for IHC-FoFr, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 39 publications.
Images
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286), expandable thumbnail](https://content.abcam.com/products/images/ssea3-antibody-mc631-ab16286--flow-cytometry-img42545.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286)")
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286), expandable thumbnail](https://content.abcam.com/products/images/ssea3-antibody-mc631-ab16286--flow-cytometry-img131053.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-SSEA3 antibody [MC631] (AB16286)")
![Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (AB16286), expandable thumbnail](https://content.abcam.com/products/images/ssea3-antibody-mc631-ab16286--immunocytochemistry-immunofluorescence-img103233.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (AB16286)")
Publications
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- Scientific reports 12:175072022High throughput screening of mesenchymal stem cell lines using deep learning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyuwon Kim,Jung Ho Jeon,Keonhyeok Park,Sung Won Kim,Do Hyun Kim,Seungchul LeePubMed 36266301
- Development, growth & differentiation 64:325-3412022Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSho Yoshimatsu,Atsushi Yamazaki,Kazuya Edamura,Yuko Koushige,Hisashi Shibuya,Emi Qian,Tsukika Sato,Junko Okahara,Noriyuki Kishi,Toshiaki Noce,Yoshifumi Yamaguchi,Hideyuki OkanoPubMed 35841539
- EMBO reports 23:e540272022Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmmar Jabali,Anne Hoffrichter,Ana Uzquiano,Fabio Marsoner,Ruven Wilkens,Marco Siekmann,Bettina Bohl,Andrea C Rossetti,Sandra Horschitz,Philipp Koch,Fiona Francis,Julia LadewigPubMed 35289477
- Biomedicines 9:2021Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlena Kashirina,Alena Gavrina,Emil Kryukov,Vadim Elagin,Yuliya Kolesova,Alexander Artyuhov,Ekaterina Momotyuk,Vepa Abdyyev,Natalia Meshcheryakova,Elena Zagaynova,Erdem Dashinimaev,Aleksandra KashinaPubMed 34829971
- Stem cell research & therapy 12:4022021Potential application of human neural crest-derived nasal turbinate stem cells for the treatment of neuropathology and impaired cognition in models of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Yeon Lim,Sang In Park,Soon A Park,Jung Ho Jeon,Ho Yong Jung,Jung-Min Yon,Sin-Soo Jeun,Hyun Kook Lim,Sung Won KimPubMed 34256823
- Cell stem cell 28:1549-1565.e122021Principles of signaling pathway modulation for enhancing human naive pluripotency induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Bayerl,Muneef Ayyash,Tom Shani,Yair Shlomo Manor,Ohad Gafni,Rada Massarwa,Yael Kalma,Alejandro Aguilera-Castrejon,Mirie Zerbib,Hadar Amir,Daoud Sheban,Shay Geula,Nofar Mor,Leehee Weinberger,Segev Naveh Tassa,Vladislav Krupalnik,Bernardo Oldak,Nir Livnat,Shadi Tarazi,Shadi Tawil,Emilie Wildschutz,Shahd Ashouokhi,Lior Lasman,Varda Rotter,Suhair Hanna,Dalit Ben-Yosef,Noa Novershtern,Sergey Viukov,Jacob H HannaPubMed 33915080
- Stem cells (Dayton, Ohio) 39:866-8812021A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair, generating improved genetically stable human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Belén Alvarez-Palomo,Jordi Requena-Osete,Raul Delgado-Morales,Victoria Moreno-Manzano,Carme Grau-Bove,Agueda M Tejera,Manel Juan Otero,Carme Barrot,Irene Santos-Barriopedro,Alejandro Vaquero,Jovita Mezquita-Pla,Sebastian Moran,Carlos Hobeich Naya,Iris Garcia-Martínez,Francisco Vidal Pérez,María A Blasco,Manel Esteller,Michael J EdelPubMed 33621399
- Laryngoscope investigative otolaryngology 5:903-9102020Heterogeneity and hierarchy of the tissue stem cells in the human newborn vocal fold mucosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiminori Sato,Shun-Ichi Chitose,Fumihiko Sato,Kiminobu Sato,Takeharu Ono,Hirohito UmenoPubMed 33134538
- Acta histochemica 121:164-1702018Differentiation potential of the cells in the macula flava of the human vocal fold mucosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumihiko Sato,Shun-Ichi Chitose,Kiminori Sato,Takashi Kurita,Kiminobu Sato,Hirohito Umeno,Hirohisa YanoPubMed 30558911
- Stem cell research 28:177-1802018Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells.
Key facts
- Isotype
- IgM
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-FoFr | Flow Cyt | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Alternative names
Stage specific embryonic antigen 3
Recommended products
Rat Monoclonal SSEA3 antibody. Suitable for IHC-FoFr, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 39 publications.
Key facts
- Isotype
- IgM
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- MC631
- Purity
- Tissue culture supernatant
- Concentration
- Purification notes
Tissue culture supernatant was cross flow concentrated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The monoclonal antibody that defines SSEA-3 was derived by fusing cells of the murine myeloma cell line SP2/0 with the spleen cells of a Fisher rat immunized with 4-8-cell-stage zonapellucida-free embryos from randomly bred mice.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SSEA3 also known as Stage-Specific Embryonic Antigen-3 is a glycosphingolipid with a molecular mass not specifically defined due to its lipid nature. This antigen is expressed on the surface of pluripotent stem cells particularly human embryonic stem cells and some germ cells. SSEA3 belongs to the globoseries of glycolipids and is widely used as a marker for undifferentiated cells. The expression of SSEA3 decreases as these cells undergo differentiation which makes it useful in studying stem cell biology and early development processes.
- Biological function summary
SSEA3 plays a role in cell adhesion and signaling during early embryogenesis. It does not form a complex itself but exists in the cell membrane contributing to interactions with other molecules or possibly engaging in signaling processes essential for cell proliferation and maintenance of stemness. The role of SSEA3 as a marker in detecting undifferentiated cells uncovers its importance in maintaining specific cell states.
- Pathways
SSEA3 may influence cellular pathways that govern stem cell maintenance and differentiation. Though not directly a pathway component it associates with proteins involved in pluripotency regulation like the Wnt and Notch pathways which are important for determining cell fate and growth. These pathways involve connections to proteins such as beta-catenin in the Wnt pathway which can influence stemness and cellular development.
- Associated diseases and disorders
Alterations in SSEA3 expression have been related to certain cancers and stem cell-related diseases. SSEA3 is sometimes found in higher levels in cancers that exhibit stem cell-like characteristics such as certain teratocarcinomas. It connects to proteins implicated in oncogenesis through shared expression patterns in tumors and stem cells like the Oct4 protein which is known for its role in maintaining stem cell properties and is often reactivated in cancer cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286), expandable thumbnail](https://content.abcam.com/products/images/ssea3-antibody-mc631-ab16286--flow-cytometry-img42545.jpg "Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)")
This image is courtesy of Prof Chris Denning's lab, University of NottinghamFlow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)
Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286), expandable thumbnail](https://content.abcam.com/products/images/ssea3-antibody-mc631-ab16286--flow-cytometry-img131053.jpg "Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)")
This image is from an anonymous collaborator.Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)
Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).
![Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286), expandable thumbnail](https://content.abcam.com/products/images/ssea3-antibody-mc631-ab16286--immunocytochemistry-immunofluorescence-img103233.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)")
This image is courtesy of the laboratory of Peter Andrews.Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)
The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286.
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