Anti-SSEA4 antibody [MC813-70]

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

Immunofluorescence analysis of Human dental pulp stem cells, staining SSEA4 with ab16287 at 1/40 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.

Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 6.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.

• Flow Cyt

Collaborator

Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (AB16287)

Human induced pluripotent stem cells (iPSCs) stained with ab16287 (red fill) with secondary only control (blue fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16287, 1/9600 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG at 1/1000 dilution for 30 min at 4°C. iPSCs differentiated for 14 days toward a cardiomyocyte lineage were used as a negative control. Acquisition of >20,000 total events were collected using a 100mW solid state diode laser (488nm) and 529/28 bandpass filter.

This image is courtesy of Professor Chris Denning's lab, University of Nottingham

• ICC/IF

AbReview15349****

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100 : in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor^® 588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.

This image is a courtesy of Fiona Lewis

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.

• Flow Cyt

Unknown

Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (AB16287)

anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.

• ICC/IF

AbReview57384****

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling SSEA4 with ab16287 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. Cells were blocked with 5% serum for 20 minutes at 25°C. A goat polyclonal anti-mouse Cy3 secondary antibody at 1/500 dilution was used.

This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany

• ICC/IF

AbReview41355****

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Sun Jeong Kim