Mouse Monoclonal SSEA4 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 180 publications.
IgG3
Mouse
Preservative: 0.02% Sodium azide
Liquid
Monoclonal
Flow Cyt | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Predicted | Predicted |
Rat | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Stage specific embryonic antigen 4
Mouse Monoclonal SSEA4 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 180 publications.
IgG3
Mouse
Preservative: 0.02% Sodium azide
Liquid
Monoclonal
MC813-70
Tissue culture supernatant
kappa
Tissue culture supernatant was cross flow concentrated.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
SSEA4 known as Stage-Specific Embryonic Antigen-4 is a glycolipid antigen involved in various cellular processes. It has a molecular mass of approximately 868.6 Da. SSEA4 is mainly expressed on the surface of pluripotent stem cells including embryonic stem cells and induced pluripotent stem cells. Additionally it can be found in germ cells and certain cancer cells serving as an important marker for these cell types.
SSEA4 plays a critical role in the identity and maintenance of pluripotency in stem cells. As a component of the glycosphingolipid complex it is involved in cell signaling pathways that regulate cell differentiation and self-renewal. The presence of SSEA4 on cell surfaces helps in identifying pluripotent stem cells facilitating research and applications in regenerative medicine.
SSEA4 influences several key cellular processes related to pluripotency and differentiation. It integrates prominently in the Wnt signaling pathway and the PI3K/Akt pathway which are essential for maintaining stem cell pluripotency and promoting survival. Interactions with proteins such as β-catenin in the Wnt pathway help modulate transcription factors critical for pluripotency.
SSEA4 expression associates with certain cancers particularly in germ cell tumors and some neuroblastomas. In cancer research it interacts with other surface antigens such as CD133 influencing tumor-initiating cell populations. The presence of SSEA4 in these cells can indicate aggressive tumor phenotypes and potential targets for therapeutic intervention.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Immunofluorescence analysis of Human dental pulp stem cells, staining SSEA4 with ab16287 at 1/40 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
Human induced pluripotent stem cells (iPSCs) stained with ab16287 (red fill) with secondary only control (blue fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16287, 1/9600 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG at 1/1000 dilution for 30 min at 4°C. iPSCs differentiated for 14 days toward a cardiomyocyte lineage were used as a negative control. Acquisition of >20,000 total events were collected using a 100mW solid state diode laser (488nm) and 529/28 bandpass filter.
anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling SSEA4 with ab16287 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. Cells were blocked with 5% serum for 20 minutes at 25°C. A goat polyclonal anti-mouse Cy3 secondary antibody at 1/500 dilution was used.
2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor® 588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.
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