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AB186730

Anti-SSPN antibody [EPR15515]

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(2 Publications)

Rabbit Recombinant Monoclonal SSPN antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

KRAG, SSPN, Sarcospan, K-ras oncogene-associated protein, Kirsten-ras-associated protein

2 Images
Western blot - Anti-SSPN antibody [EPR15515] (AB186730)
  • WB

Supplier Data

Western blot - Anti-SSPN antibody [EPR15515] (AB186730)

All lanes:

Western blot - Anti-SSPN antibody [EPR15515] (ab186730) at 1/10000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

A673 cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

false

Western blot - Anti-SSPN antibody [EPR15515] (AB186730)
  • WB

Supplier Data

Western blot - Anti-SSPN antibody [EPR15515] (AB186730)

All lanes:

Western blot - Anti-SSPN antibody [EPR15515] (ab186730) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat heart tissue lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 27 kDa

false

  • Carrier free

    Anti-SSPN antibody [EPR15515] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15515

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin (SSPN) is a protein that functions mechanically as a cell membrane protein. It appears widely in various tissues but notably in skeletal muscle and the central nervous system. The approximate molecular mass of SSPN is 25 kDa. Commonly one finds it as a part of membrane structures in cells where it plays roles in maintaining membrane integrity and facilitating signal transduction.
Biological function summary

SSPN associates with the dystrophin-glycoprotein complex which plays important roles in the structural linkage between the extracellular matrix and the cytoskeleton. This association contributes to cellular stability and muscle function. SSPN is important for cellular communication impacting signal transduction processes and maintaining cellular architecture.

Pathways

The dystrophin-glycoprotein complex that includes SSPN is key in the mechanotransduction pathway where it translates mechanical stimuli into chemical signals. Additionally SSPN interacts with proteins like dystrophin helping transmit mechanical tension in the muscle tissue and caveolin which plays roles in signal transduction pathways. These interactions are pivotal for muscular responses and cellular signaling adaptations.

Mutations or disruptions in SSPN function link closely to muscular dystrophies such as Duchenne and Becker muscular dystrophies. These conditions also involve related proteins such as dystrophin highlighting the importance of SSPN in muscle health and disease. Moreover SSPN's association with the central nervous system suggests relevancy in neurodegenerative disorders although further research is needed to fully understand these connections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.
See full target information SSPN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed31689918

2019

Lack of Delta-Sarcoglycan () Results in Retinal Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Andric C Perez-Ortiz,Martha J Peralta-Ildefonso,Esmeralda Lira-Romero,Ernesto Moya-Albor,Jorge Brieva,Israel Ramirez-Sanchez,Carmen Clapp,Alexandra Luna-Angulo,Alvaro Rendon,Elva Adan-Castro,Gabriela Ramírez-Hernández,Nundehui Díaz-Lezama,Ramón M Coral-Vázquez,Francisco J Estrada-Mena

Human molecular genetics 24:5234-49 PubMed26123487

2015

FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuko Kato,Kris G Alavattam,Ho-Su Sin,Amom Ruhikanta Meetei,Qishen Pang,Paul R Andreassen,Satoshi H Namekawa
View all publications
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Product promise

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