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AB250890

Anti-SSPN antibody [EPR15515] - BSA and Azide free

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Rabbit Recombinant Monoclonal SSPN antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

View Alternative Names

KRAG, SSPN, Sarcospan, K-ras oncogene-associated protein, Kirsten-ras-associated protein

2 Images
Western blot - Anti-SSPN antibody [EPR15515] - BSA and Azide free (AB250890)
  • WB

Supplier Data

Western blot - Anti-SSPN antibody [EPR15515] - BSA and Azide free (AB250890)

This data was developed using ab186730, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SSPN antibody [EPR15515] (<a href='/en-us/products/primary-antibodies/sspn-antibody-epr15515-ab186730'>ab186730</a>) at 1/10000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

A673 cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

false

Western blot - Anti-SSPN antibody [EPR15515] - BSA and Azide free (AB250890)
  • WB

Supplier Data

Western blot - Anti-SSPN antibody [EPR15515] - BSA and Azide free (AB250890)

This data was developed using ab186730, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SSPN antibody [EPR15515] (<a href='/en-us/products/primary-antibodies/sspn-antibody-epr15515-ab186730'>ab186730</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat heart tissue lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15515

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250890 is the carrier-free version of ab186730.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin (SSPN) is a protein that functions mechanically as a cell membrane protein. It appears widely in various tissues but notably in skeletal muscle and the central nervous system. The approximate molecular mass of SSPN is 25 kDa. Commonly one finds it as a part of membrane structures in cells where it plays roles in maintaining membrane integrity and facilitating signal transduction.
Biological function summary

SSPN associates with the dystrophin-glycoprotein complex which plays important roles in the structural linkage between the extracellular matrix and the cytoskeleton. This association contributes to cellular stability and muscle function. SSPN is important for cellular communication impacting signal transduction processes and maintaining cellular architecture.

Pathways

The dystrophin-glycoprotein complex that includes SSPN is key in the mechanotransduction pathway where it translates mechanical stimuli into chemical signals. Additionally SSPN interacts with proteins like dystrophin helping transmit mechanical tension in the muscle tissue and caveolin which plays roles in signal transduction pathways. These interactions are pivotal for muscular responses and cellular signaling adaptations.

Mutations or disruptions in SSPN function link closely to muscular dystrophies such as Duchenne and Becker muscular dystrophies. These conditions also involve related proteins such as dystrophin highlighting the importance of SSPN in muscle health and disease. Moreover SSPN's association with the central nervous system suggests relevancy in neurodegenerative disorders although further research is needed to fully understand these connections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.
See full target information SSPN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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