AB121458

Anti-SSR3 antibody

Name: Anti-SSR3 antibody (ab121458) | Abcam
Brand: Abcam Limited
SKU: AB121458
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SSR3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SSR3 aa 50-150.

View Alternative Names

TRAPG, SSR3, Translocon-associated protein subunit gamma, TRAP-gamma, Signal sequence receptor subunit gamma, SSR-gamma

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse choroid plexus tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SSR3 antibody (AB121458)

All lanes:

Western blot - Anti-SSR3 antibody (ab121458) at 0.4 µg/mL

All lanes:

RPMI-8226 (human lymphoblast cell line) cell lysate

Predicted band size: 21 kDa

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Supplier Data

Western blot - Anti-SSR3 antibody (AB121458)

All lanes:

Western blot - Anti-SSR3 antibody (ab121458) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat urinary bladder tumor cell line) cell lysate

Predicted band size: 21 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SSR3 antibody (AB121458)

All lanes:

Western blot - Anti-SSR3 antibody (ab121458) at 0.4 µg/mL

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-SSR3 antibody (AB121458)

All lanes:

Western blot - Anti-SSR3 antibody (ab121458) at 0.4 µg/mL

All lanes:

Mouse cerebellum tissue lysate

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SSR3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UNL2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSR3 also known as the signal sequence receptor subunit 3 or translocon-associated protein delta subunit is a component of the protein translocon complex located in the endoplasmic reticulum (ER) membrane. The protein has a mass of approximately 18-20 kDa. SSR3 is expressed in various tissues with significant levels in metabolically active tissues such as the liver and kidney. As a membrane-associated protein it functions as part of a larger complex that assists in the translocation of nascent polypeptides into the ER lumen.

Biological function summary

The involvement of SSR3 in the protein translocation process emphasizes its role in maintaining ER function and protein handling. It forms part of the heterotetrameric translocon-associated protein (TRAP) complex which includes SSR1 SSR2 and SSR4. This complex operates in the early stages of the secretory pathway assisting in the proper insertion and folding of nascent proteins into the ER. Consequently SSR3 plays a direct role in the synthesis of secretory and membrane-bound proteins ensuring the fidelity of protein modification and trafficking within the cell.

Pathways

SSR3 is intricately linked with the secretory pathway important for cell function and communication. The protein directly interacts with the translocon machinery which is responsible for mediating the transfer of nascent polypeptides through the ER membrane. SSR3's function in conjunction with TRAP and other components such as the signal recognition particle receptor allow transition into additional cellular pathways like the unfolded protein response (UPR). This relationship maintains ER homeostasis and cellular adaptability situating SSR3 within a network of proteins that respond to ER stress conditions.

SSR3 has connections to conditions related to ER stress such as neurodegenerative diseases and certain forms of diabetes. Given its role in protein folding and quality control disruptions in SSR3 function can contribute to the accumulation of misfolded proteins possibly linking it to disorders like Alzheimer's disease. In these scenarios interactions with proteins involved in the UPR like ATF6 and PERK indicate SSR3's contribution to the cellular attempt to rectify protein misfolding and restore ER capacity. Therefore understanding SSR3's function and regulation provides insight into the cellular response to stress that could influence therapeutic strategies for related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.

See full target information SSR3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human pathology 81:47-54 PubMed29940286

2018

Overexpression of signal sequence receptor γ predicts poor survival in patients with hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shanzhou Huang,Wenqiang Zhong,Zhi Shi,Kun Wang,Huilin Jin,Zijian Zhang,Huanyu Wang,Yongcheng Wei,Sixv Chen,Qi Zhou,Xiaoshun He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSR3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

Western blot - Anti-SSR3 antibody (AB121458)

Western blot - Anti-SSR3 antibody (AB121458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody (AB121458)

Western blot - Anti-SSR3 antibody (AB121458)

Western blot - Anti-SSR3 antibody (AB121458)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise