Anti-SSR3 antibody - N-terminal

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936)

Immunohistochemical analysis of paraffin-embedded Human prostatic cancer tissue labeling SSR3 with ab190936 at 1 μg/ml.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SSR3 antibody - N-terminal (AB190936)

SSR3 was detected in immunocytochemical section of HeLa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936)

Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling SSR3 with ab190936 at 1 μg/ml.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936)

SSR3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936)

Immunohistochemical analysis of paraffin-embedded Rat intestine tissue labeling SSR3 with ab190936 at 1 μg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936)

SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936)

SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• WB

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Western blot - Anti-SSR3 antibody - N-terminal (AB190936)

All lanes:

Western blot - Anti-SSR3 antibody - N-terminal (ab190936) at 0.5 µg/mL

Lane 1:

Rat liver tissue lysate

Lane 2:

Rat spleen tissue lysate

Lane 3:

A431 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

U87 cell lysate

Lane 6:

SMMC cell lysate

Predicted band size: 21 kDa

false