Rabbit Polyclonal SSR3 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SSR3 aa 1-50.
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- Nature communications 11:20322020Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Bilska et. al.PubMed 32341344
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SSR3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNL2
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.
Alternative names
Translocon-associated protein subunit gamma, TRAP-gamma, Signal sequence receptor subunit gamma, SSR-gamma, TRAPG, SSR3
Recommended products
Rabbit Polyclonal SSR3 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SSR3 aa 1-50.
Alternative names
Translocon-associated protein subunit gamma, TRAP-gamma, Signal sequence receptor subunit gamma, SSR-gamma, TRAPG, SSR3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SSR3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNL2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SSR3 also known as the signal sequence receptor subunit 3 or translocon-associated protein delta subunit is a component of the protein translocon complex located in the endoplasmic reticulum (ER) membrane. The protein has a mass of approximately 18-20 kDa. SSR3 is expressed in various tissues with significant levels in metabolically active tissues such as the liver and kidney. As a membrane-associated protein it functions as part of a larger complex that assists in the translocation of nascent polypeptides into the ER lumen.
- Biological function summary
The involvement of SSR3 in the protein translocation process emphasizes its role in maintaining ER function and protein handling. It forms part of the heterotetrameric translocon-associated protein (TRAP) complex which includes SSR1 SSR2 and SSR4. This complex operates in the early stages of the secretory pathway assisting in the proper insertion and folding of nascent proteins into the ER. Consequently SSR3 plays a direct role in the synthesis of secretory and membrane-bound proteins ensuring the fidelity of protein modification and trafficking within the cell.
- Pathways
SSR3 is intricately linked with the secretory pathway important for cell function and communication. The protein directly interacts with the translocon machinery which is responsible for mediating the transfer of nascent polypeptides through the ER membrane. SSR3's function in conjunction with TRAP and other components such as the signal recognition particle receptor allow transition into additional cellular pathways like the unfolded protein response (UPR). This relationship maintains ER homeostasis and cellular adaptability situating SSR3 within a network of proteins that respond to ER stress conditions.
- Associated diseases and disorders
SSR3 has connections to conditions related to ER stress such as neurodegenerative diseases and certain forms of diabetes. Given its role in protein folding and quality control disruptions in SSR3 function can contribute to the accumulation of misfolded proteins possibly linking it to disorders like Alzheimer's disease. In these scenarios interactions with proteins involved in the UPR like ATF6 and PERK indicate SSR3's contribution to the cellular attempt to rectify protein misfolding and restore ER capacity. Therefore understanding SSR3's function and regulation provides insight into the cellular response to stress that could influence therapeutic strategies for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling SSR3 with ab190936 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunocytochemistry/ Immunofluorescence - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in immunocytochemical section of HeLa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Western blot - Anti-SSR3 antibody - N-terminal (ab190936)
All lanes: Western blot - Anti-SSR3 antibody - N-terminal (ab190936) at 0.5 µg/mL
Lane 1: Rat liver tissue lysate
Lane 2: Rat spleen tissue lysate
Lane 3: A431 cell lysate
Lane 4: HeLa cell lysate
Lane 5: U87 cell lysate
Lane 6: SMMC cell lysate
Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
Immunohistochemical analysis of paraffin-embedded Human prostatic cancer tissue labeling SSR3 with ab190936 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
Immunohistochemical analysis of paraffin-embedded Rat intestine tissue labeling SSR3 with ab190936 at 1 μg/ml.
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