Anti-SSR3 antibody - N-terminal (ab190936)

Rabbit Polyclonal SSR3 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SSR3 aa 1-50.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (AB190936), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
Form: Liquid
Clonality: Polyclonal

Immunogen

Synthetic Peptide within Human SSR3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNL2

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF
Human	Tested	Expected	Tested
Mouse	Tested	Expected	Expected
Rat	Tested	Tested	Expected
Cow	Predicted	Predicted	Predicted
Orangutan	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.50000-1.00000 µg/mL	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 0.50000-1.00000 µg/mL	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 0.50000-1.00000 µg/mL	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Cow, Orangutan	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 0.10000-0.50000 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cow, Orangutan	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 0.50000-1.00000 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cow, Orangutan	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

See full target information SSR3

Function

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.

Alternative names

TRAPG, SSR3, Translocon-associated protein subunit gamma, TRAP-gamma, Signal sequence receptor subunit gamma, SSR-gamma

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
Form: Liquid
Clonality: Polyclonal
Immunogen: Synthetic Peptide within Human SSR3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNL2
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SSR3 also known as the signal sequence receptor subunit 3 or translocon-associated protein delta subunit is a component of the protein translocon complex located in the endoplasmic reticulum (ER) membrane. The protein has a mass of approximately 18-20 kDa. SSR3 is expressed in various tissues with significant levels in metabolically active tissues such as the liver and kidney. As a membrane-associated protein it functions as part of a larger complex that assists in the translocation of nascent polypeptides into the ER lumen.

Biological function summary

The involvement of SSR3 in the protein translocation process emphasizes its role in maintaining ER function and protein handling. It forms part of the heterotetrameric translocon-associated protein (TRAP) complex which includes SSR1 SSR2 and SSR4. This complex operates in the early stages of the secretory pathway assisting in the proper insertion and folding of nascent proteins into the ER. Consequently SSR3 plays a direct role in the synthesis of secretory and membrane-bound proteins ensuring the fidelity of protein modification and trafficking within the cell.

Pathways

SSR3 is intricately linked with the secretory pathway important for cell function and communication. The protein directly interacts with the translocon machinery which is responsible for mediating the transfer of nascent polypeptides through the ER membrane. SSR3's function in conjunction with TRAP and other components such as the signal recognition particle receptor allow transition into additional cellular pathways like the unfolded protein response (UPR). This relationship maintains ER homeostasis and cellular adaptability situating SSR3 within a network of proteins that respond to ER stress conditions.

Associated diseases and disorders

SSR3 has connections to conditions related to ER stress such as neurodegenerative diseases and certain forms of diabetes. Given its role in protein folding and quality control disruptions in SSR3 function can contribute to the accumulation of misfolded proteins possibly linking it to disorders like Alzheimer's disease. In these scenarios interactions with proteins involved in the UPR like ATF6 and PERK indicate SSR3's contribution to the cellular attempt to rectify protein misfolding and restore ER capacity. Therefore understanding SSR3's function and regulation provides insight into the cellular response to stress that could influence therapeutic strategies for related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling SSR3 with ab190936 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Immunocytochemistry/ Immunofluorescence - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in immunocytochemical section of HeLa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Western blot - Anti-SSR3 antibody - N-terminal (ab190936)
All lanes: Western blot - Anti-SSR3 antibody - N-terminal (ab190936) at 0.5 µg/mL
Lane 1: Rat liver tissue lysate
Lane 2: Rat spleen tissue lysate
Lane 3: A431 cell lysate
Lane 4: HeLa cell lysate
Lane 5: U87 cell lysate
Lane 6: SMMC cell lysate
Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
Immunohistochemical analysis of paraffin-embedded Human prostatic cancer tissue labeling SSR3 with ab190936 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
Immunohistochemical analysis of paraffin-embedded Rat intestine tissue labeling SSR3 with ab190936 at 1 μg/ml.