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AB129109

Anti-SSRP1 antibody [EPR7894]

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Rabbit Recombinant Monoclonal SSRP1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

FACT80, SSRP1, FACT complex subunit SSRP1, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, Recombination signal sequence recognition protein 1, Structure-specific recognition protein 1, T160, FACT 80 kDa subunit, FACTp80, hSSRP1

3 Images
Flow Cytometry (Intracellular) - Anti-SSRP1 antibody [EPR7894] (AB129109)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SSRP1 antibody [EPR7894] (AB129109)

ab129109, at 1/100 dilution, staining SSRP1 in permeabilized MOLT4 cells by intracellular flow cytometry (red) or Rabbit IgG negative control (green).

Western blot - Anti-SSRP1 antibody [EPR7894] (AB129109)
  • WB

Unknown

Western blot - Anti-SSRP1 antibody [EPR7894] (AB129109)

All lanes:

Western blot - Anti-SSRP1 antibody [EPR7894] (ab129109) at 1/10000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MOLT4 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 81 kDa

false

OI-RD Scanning - Anti-SSRP1 antibody [EPR7894] (AB129109)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SSRP1 antibody [EPR7894] (AB129109)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SSRP1 antibody [EPR7894] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7894

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSRP1 also known as Structure-Specific Recognition Protein 1 has an important role in DNA-related processes. This protein with a molecular weight of about 80 kDa binds DNA to regulate its structure during transcription and replication. SSRP1 localizes in the nucleus and expresses widely across various tissues indicating its broad functional significance. SSRP1 often pairs with SPT16 to form the FACT (Facilitates Chromatin Transcription) complex which aids in nucleosome disassembly and reassembly.
Biological function summary

SSRP1 is integral to processes involving chromatin reorganization and transcriptional regulation. It operates as a component of the FACT complex facilitating the modification of chromatin structure enabling access to the underlying DNA for active transcription. By altering nucleosome structure it assists RNA polymerase II in transcribing through chromatin directly influencing gene expression regulation.

Pathways

SSRP1 participates actively in DNA transcription and repair mechanisms. It is an essential player in the transcription elongation pathway interacting with RNA polymerase II. Additionally it influences the DNA repair pathways working in concert with proteins such as SPT16 and histones to maintain genomic stability. Its function ensures efficient transcription and protection against DNA damage.

SSRP1 connections emerge in the context of cancer and Alzheimer's disease. In cancer SSRP1 expression levels can correlate with tumor progression influencing the behavior of cancer cells through its role in transcriptional regulation. In Alzheimer's alterations in chromatin structure involving SSRP1 may link to disease progression. The activity of SSRP1 interacts with proteins such as p53 in cancer highlighting its role in disease mechanisms and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
See full target information SSRP1
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Product promise

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