AB137034

Anti-SSRP1 antibody [EPR7895]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal SSRP1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

FACT80, SSRP1, FACT complex subunit SSRP1, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, Recombination signal sequence recognition protein 1, Structure-specific recognition protein 1, T160, FACT 80 kDa subunit, FACTp80, hSSRP1

2 Images

Lab

Western blot - Anti-SSRP1 antibody [EPR7895] (AB137034)

All lanes:

Western blot - Anti-SSRP1 antibody [EPR7895] (ab137034) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Molt-4 (Human lymphoblastic leukemia tlymphoblast) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 81 kDa

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Lab

Western blot - Anti-SSRP1 antibody [EPR7895] (AB137034)

All lanes:

Western blot - Anti-SSRP1 antibody [EPR7895] (ab137034) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa,81 kDa

Observed band size: 65 kDa,81 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7895

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSRP1 also known as Structure-Specific Recognition Protein 1 has an important role in DNA-related processes. This protein with a molecular weight of about 80 kDa binds DNA to regulate its structure during transcription and replication. SSRP1 localizes in the nucleus and expresses widely across various tissues indicating its broad functional significance. SSRP1 often pairs with SPT16 to form the FACT (Facilitates Chromatin Transcription) complex which aids in nucleosome disassembly and reassembly.

Biological function summary

SSRP1 is integral to processes involving chromatin reorganization and transcriptional regulation. It operates as a component of the FACT complex facilitating the modification of chromatin structure enabling access to the underlying DNA for active transcription. By altering nucleosome structure it assists RNA polymerase II in transcribing through chromatin directly influencing gene expression regulation.

Pathways

SSRP1 participates actively in DNA transcription and repair mechanisms. It is an essential player in the transcription elongation pathway interacting with RNA polymerase II. Additionally it influences the DNA repair pathways working in concert with proteins such as SPT16 and histones to maintain genomic stability. Its function ensures efficient transcription and protection against DNA damage.

SSRP1 connections emerge in the context of cancer and Alzheimer's disease. In cancer SSRP1 expression levels can correlate with tumor progression influencing the behavior of cancer cells through its role in transcriptional regulation. In Alzheimer's alterations in chromatin structure involving SSRP1 may link to disease progression. The activity of SSRP1 interacts with proteins such as p53 in cancer highlighting its role in disease mechanisms and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.

See full target information SSRP1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105538 PubMed38072046

2023

Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.

Applications

Unspecified application

Species

Unspecified reactive species

Peijun Wang,Na Fan,Wanting Yang,Pengbo Cao,Guojun Liu,Qi Zhao,Pengfei Guo,Xihe Li,Xinhua Lin,Ning Jiang,Buhe Nashun

PLoS pathogens 18:e1010353 PubMed35395063

2022

Parvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Salla Mattola,Kari Salokas,Vesa Aho,Elina Mäntylä,Sami Salminen,Satu Hakanen,Einari A Niskanen,Julija Svirskaite,Teemu O Ihalainen,Kari J Airenne,Minna Kaikkonen-Määttä,Colin R Parrish,Markku Varjosalo,Maija Vihinen-Ranta

The Journal of biological chemistry 297:101360 PubMed34756889

2021

Structural insights into multifunctionality of human FACT complex subunit hSSRP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehui Li,Huiyan Li,Qian Jing,Mengxue Wang,Tingting Hu,Li Li,Qiuping Zhang,Mengxin Liu,Yu Vincent Fu,Junhong Han,Dan Su

Nucleic acids research 48:10211-10225 PubMed32894293

2020

Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Fuquan Chen,Weiyu Zhang,Dan Xie,Tingting Gao,Zhiqiang Dong,Xinyi Lu

Journal of virology 91: PubMed28122976

2017

The FACT Complex Promotes Avian Leukosis Virus DNA Integration.

Applications

Unspecified application

Species

Unspecified reactive species

Shelby Winans,Ross C Larue,Carly M Abraham,Nikolozi Shkriabai,Amelie Skopp,Duane Winkler,Mamuka Kvaratskhelia,Karen L Beemon

View all publications

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