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AB240097

Anti-SSRP1 antibody [EPR7895] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SSRP1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

FACT80, SSRP1, FACT complex subunit SSRP1, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, Recombination signal sequence recognition protein 1, Structure-specific recognition protein 1, T160, FACT 80 kDa subunit, FACTp80, hSSRP1

2 Images
Western blot - Anti-SSRP1 antibody [EPR7895] - BSA and Azide free (AB240097)
  • WB

Lab

Western blot - Anti-SSRP1 antibody [EPR7895] - BSA and Azide free (AB240097)

This data was developed using ab137034, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SSRP1 antibody [EPR7895] (<a href='/en-us/products/primary-antibodies/ssrp1-antibody-epr7895-ab137034'>ab137034</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Molt-4 (Human lymphoblastic leukemia tlymphoblast) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 81 kDa

false

Western blot - Anti-SSRP1 antibody [EPR7895] - BSA and Azide free (AB240097)
  • WB

Lab

Western blot - Anti-SSRP1 antibody [EPR7895] - BSA and Azide free (AB240097)

This data was developed using ab137034, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SSRP1 antibody [EPR7895] (<a href='/en-us/products/primary-antibodies/ssrp1-antibody-epr7895-ab137034'>ab137034</a>) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa,81 kDa

Observed band size: 65 kDa,81 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7895

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab240097 is the carrier-free version of ab137034.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSRP1 also known as Structure-Specific Recognition Protein 1 has an important role in DNA-related processes. This protein with a molecular weight of about 80 kDa binds DNA to regulate its structure during transcription and replication. SSRP1 localizes in the nucleus and expresses widely across various tissues indicating its broad functional significance. SSRP1 often pairs with SPT16 to form the FACT (Facilitates Chromatin Transcription) complex which aids in nucleosome disassembly and reassembly.
Biological function summary

SSRP1 is integral to processes involving chromatin reorganization and transcriptional regulation. It operates as a component of the FACT complex facilitating the modification of chromatin structure enabling access to the underlying DNA for active transcription. By altering nucleosome structure it assists RNA polymerase II in transcribing through chromatin directly influencing gene expression regulation.

Pathways

SSRP1 participates actively in DNA transcription and repair mechanisms. It is an essential player in the transcription elongation pathway interacting with RNA polymerase II. Additionally it influences the DNA repair pathways working in concert with proteins such as SPT16 and histones to maintain genomic stability. Its function ensures efficient transcription and protection against DNA damage.

SSRP1 connections emerge in the context of cancer and Alzheimer's disease. In cancer SSRP1 expression levels can correlate with tumor progression influencing the behavior of cancer cells through its role in transcriptional regulation. In Alzheimer's alterations in chromatin structure involving SSRP1 may link to disease progression. The activity of SSRP1 interacts with proteins such as p53 in cancer highlighting its role in disease mechanisms and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
See full target information SSRP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:770-783 PubMed38600236

2024

Transcription-coupled DNA-protein crosslink repair by CSB and CRL4-mediated degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Marjolein van Sluis,Qing Yu,Melanie van der Woude,Camila Gonzalo-Hansen,Shannon C Dealy,Roel C Janssens,Hedda B Somsen,Anisha R Ramadhin,Dick H W Dekkers,Hannah Lena Wienecke,Joris J P G Demmers,Anja Raams,Carlota Davó-Martínez,Diana A Llerena Schiffmacher,Marvin van Toorn,David Häckes,Karen L Thijssen,Di Zhou,Judith G Lammers,Alex Pines,Wim Vermeulen,Joris Pothof,Jeroen A A Demmers,Debbie L C van den Berg,Hannes Lans,Jurgen A Marteijn
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com