Anti-SSTR3 antibody [HL2681] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

ab325874 detects Somatostatin receptor 3 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. Somatostatin receptor 3 stained by ab325874 diluted at 1 : 1200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

ab325874 detects Somatostatin receptor 3 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. Somatostatin receptor 3 stained by ab325874 diluted at 1 : 1200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

ab325874 detects Somatostatin receptor 3 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse cerebellum. Green : Somatostatin receptor 3 stained by ab325874 diluted at 1 : 100. Red : beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

ab325874 detects Somatostatin receptor 3 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse hippocampus. Somatostatin receptor 3 stained by ab325874 diluted at 1 : 1200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

ab325874 detects Somatostatin receptor 3 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded mouse cerebellum. Somatostatin receptor 3 stained by ab325874 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325874 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (ab325874) at 1/500 dilution

All lanes:

Human testis tissue extract at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with ab325874 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (ab325874) at 1/1000 dilution

All lanes:

Human testis tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325874 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (ab325874) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged SSTR3 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged SSTR3 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (AB325874)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325874 diluted at 1 : 5000 and competitor's antibody diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SSTR3 antibody [HL2681] - BSA and Azide free (ab325874) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged SSTR transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged SSTR transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false