Rabbit Polyclonal SSTR4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SSTR4 aa 350 to C-terminus.
View Alternative Names
Somatostatin receptor type 4, SS-4-R, SS4-R, SS4R, SST4, SSTR4
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-SSTR4 antibody (AB28578)
Immunofluorescent analysis of SSTR4 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a ab28578 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-SSTR4 antibody (AB28578)
ICC/IF image of ab28578 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28578, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-SSTR4 antibody (AB28578)
Immunocytochemistry/ Immunofluorescence analysis of 70% confluent log phase SH-SY5Y cells labeling SSTR4 with ab28578. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ab28578 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin 1/300. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-SSTR4 antibody (AB28578)
Immunofluorescent analysis of SSTR4 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab285781 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Reactivity data
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of SSTR4 involves regulating neurotransmission cell proliferation and secretion processes. It does not form part of a larger receptor complex but plays an important role in mediating inhibitory signals from somatostatin. SSTR4 modulates the release of many hormones and neurotransmitters influencing neural and endocrine communications in the body.
Pathways
SSTR4 is involved in important signaling pathways such as the somatostatin receptor signaling pathway and the MAPK signaling pathway. It often cooperates with related somatostatin receptor family members like SSTR2 and SSTR5. Through these pathways SSTR4 helps modulate cellular responses including gene expression and cell cycle control.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (6)
Recent publications for all applications. Explore the full list and refine your search
Cancers 16: PubMed39272962
2024
Applications
Unspecified application
Species
Unspecified reactive species
Journal of neuroendocrinology 34:e13109 PubMed35304807
2022
Applications
Unspecified application
Species
Unspecified reactive species
Drug design, development and therapy 15:2385-2399 PubMed34103899
2021
Applications
Unspecified application
Species
Unspecified reactive species
Brain structure & function 225:387-401 PubMed31873798
2019
Applications
Unspecified application
Species
Unspecified reactive species
Oncology letters 17:1723-1731 PubMed30675231
2019
Applications
Unspecified application
Species
Unspecified reactive species
Experimental and therapeutic medicine 16:3777-3784 PubMed30405748
2018
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Primary Antibodies
AB134152
Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
BOND RX™ Validated|RabMAb|Recombinant|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)](https://content.abcam.com/products/images/somatostatin-receptor-2-antibody-umb1-c-terminal-ab134152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417177.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com