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Rabbit Recombinant Monoclonal SSTR4 antibody. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Human, Human samples.

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Images

Western blot - Anti-SSTR4 antibody [EPR29682-48] (AB324103), expandable thumbnail
  • Indirect ELISA - Anti-SSTR4 antibody [EPR29682-48] (AB324103), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (AB324103), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAIHC-PWBICC/IF
Human
Expected
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
15.625 ng/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Receptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.

Alternative names

Somatostatin receptor type 4, SS-4-R, SS4-R, SS4R, SST4, SSTR4

Rabbit Recombinant Monoclonal SSTR4 antibody. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29682-48
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SSTR4 antibody [EPR29682-48] (ab324103), expandable thumbnail

    Western blot - Anti-SSTR4 antibody [EPR29682-48] (ab324103)

    SSTR4 Western blot staining using rabbit Anti-SSTR4 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: colon, liver, lung, testis.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10804219, PMID: 34884783).

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SSTR4 antibody [EPR29682-48] (ab324103) at 1/1000 dilution

    Lane 1: Human striatum tissue lysate at 48 µg

    Lane 2: Human colon tissue lysate at 48 µg

    Lane 3: Human liver tissue lysate at 48 µg

    Lane 4: Human thalamus tissue lysate at 48 µg

    Lane 5: Human lung tissue lysate at 48 µg

    Lane 6: Human testis tissue lysate at 48 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 42-50 kDa, 36 kDa

    Exposure time: 136s

  • Indirect ELISA - Anti-SSTR4 antibody [EPR29682-48] (ab324103), expandable thumbnail

    Indirect ELISA - Anti-SSTR4 antibody [EPR29682-48] (ab324103)

    Indirect ELISA analysis of ab324103 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.

    Antigen: Human SSTR4, Human SSTR1.

    Antigen concentration: 1000 ng/ml

    This antibody does not cross-react with human SSTR1.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103)

    SSTR4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human lung using rabbit Anti-SSTR4 antibody

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human lung.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103)

    SSTR4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon using rabbit Anti-SSTR4 antibody

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human colon.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103)

    SSTR4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human astrocytoma using rabbit Anti-SSTR4 antibody

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human astrocytoma.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSTR4 antibody [EPR29682-48] (ab324103)

    SSTR4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-SSTR4 antibody

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SSTR4 with ab324103 at 1/100 (5.14 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human cerebrum.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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