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AB325875

Anti-SSTR5 antibody [HL2756] - BSA and Azide free

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Rabbit Recombinant Monoclonal Somatostatin Receptor 5 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples. Immunogen corresponding to Synthetic Peptide within Human SSTR5.

View Alternative Names

Somatostatin receptor type 5, SS-5-R, SS5-R, SS5R, SST5, SSTR5

3 Images
Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (AB325875)
  • WB

Supplier Data

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (AB325875)

Unboiled HT-29 cytosol extract and membrane extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325875 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (ME : membrane extract; CE : cytosol extract)

All lanes:

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (ab325875) at 1/1000 dilution

Lane 1:

Unboiled HT-29 cytosol extract at 30 µg

Lane 2:

Unboiled HT-29 membrane extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (AB325875)
  • WB

Supplier Data

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (AB325875)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with ab325875 diluted at 1 : 5000 and competitor's antibody diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (ab325875) at 1/5000 dilution

Lane 1:

Non-transfected (–) unboiled 293T whole cell extracts

Lane 2:

DDDDK-tagged SSTR1 transfected (+) unboiled 293T whole cell extracts

Lane 3:

DDDDK-tagged SSTR2 transfected (+) unboiled 293T whole cell extracts

Lane 4:

DDDDK-tagged SSTR3 transfected (+) unboiled 293T whole cell extracts

Lane 5:

DDDDK-tagged SSTR4 transfected (+) unboiled 293T whole cell extracts

Lane 6:

DDDDK-tagged SSTR5 transfected (+) unboiled 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (AB325875)
  • WB

Supplier Data

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (AB325875)

Unboiled non-transfected (–) and transfected (+) 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325875 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SSTR5 antibody [HL2756] - BSA and Azide free (ab325875) at 1/5000 dilution

Lanes 1 and 5:

Unboiled non-transfected (–) Expi293 whole cell extracts at 30 µg

Lane 2:

Unboiled SSTR1 transfected (+) Expi293 whole cell extracts at 30 µg

Lanes 3, 7 and 9:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled SSTR2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled SSTR3 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 8:

Unboiled SSTR4 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled SSTR5 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2756

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SSTR5.

P35346

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Increases cell growth inhibition activity of SSTR2 following heterodimerization.
See full target information SSTR5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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