AB229054

Anti-Ssu72 antibody

Name: Anti-Ssu72 antibody (ab229054) | Abcam
Brand: Abcam Limited
SKU: AB229054

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(1 Publication)

Rabbit Polyclonal SSU72 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SSU72.

View Alternative Names

HSPC182, PNAS-120, SSU72, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, CTD phosphatase SSU72

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ssu72 antibody (AB229054)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ssu72 antibody (AB229054)

Paraffin-embedded rat liver tissue stained for Ssu72 with ab229054 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Ssu72 antibody (AB229054)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ssu72 antibody (ab229054) at 1/1000 dilution

All lanes:

Mouse placenta tissue extract at 50 µg

Predicted band size: 23 kDa

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Supplier Data

Western blot - Anti-Ssu72 antibody (AB229054)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ssu72 antibody (ab229054) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 23 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SSU72. The exact immunogen used to generate this antibody is proprietary information.

Q9NP77

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ssu72 also known as SSU72 homolog RNA polymerase II CTD phosphatase is an enzyme with a mass of approximately 24 kDa. It functions as a specific phosphatase that targets the C-terminal domain (CTD) of RNA polymerase II. Ssu72 performs its mechanical role by removing phosphate groups from serine 5 residues within the heptad repeats of the CTD which is important for the transcription cycle. The expression of Ssu72 occurs in the nucleus where its activity aligns with its role in transcription regulation.

Biological function summary

The enzymatic function of Ssu72 contributes to gene expression regulation. It plays a significant role during the transition between the transcription initiation and elongation phases. Ssu72 is an integral part of the transcription machinery and is known to associate with the cleavage and polyadenylation complex highlighting its involvement in the pre-mRNA processing. Additionally Ssu72's phosphatase activity helps regulate the interaction between RNA polymerase II and multiple transcription factors and complexes emphasizing its role in maintaining efficient transcription and RNA processing dynamics.

Pathways

Ssu72 functions within the transcription regulation pathway where it interacts with other critical transcription-related proteins such as the Symplekin and TFIIB. Its involvement in the regulation of RNA polymerase II activity connects it to RNA processing pathways including mRNA capping splicing and termination processes. These interactions facilitate the precise control of gene expression by modulating transcription elongation and mRNA processing events ensuring coordinated transcriptional responses.

The dysfunction of Ssu72 can lead to transcriptional dysregulation contributing to cancer and other transcription-associated disorders. Disruptions in its phosphatase activity may have downstream effects on RNA polymerase II and the associated transcription regulation processes. Ssu72's connection to cancer is further supported by its interaction with proteins like PinX1 a negative regulator of telomerase where altered expression or activity could influence cellular proliferation and stability potentially driving oncogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.

See full target information SSU72

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin 40:421-438 PubMed37864744

2023

Inflammatory Factor IL1α Induces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca/CaMK2b Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xia,Lu Ding,Changlin Zhang,Qi Xu,Ming Shi,Tianshun Gao,Feng-Quan Zhou,David Y B Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ssu72 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ssu72 antibody (AB229054)

Western blot - Anti-Ssu72 antibody (AB229054)

Western blot - Anti-Ssu72 antibody (AB229054)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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