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AB236415

Anti-SSX2 antibody [OTI4A11]

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(1 Publication)

Mouse Monoclonal SSX2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SSX2.

View Alternative Names

SSX2A, SSX2B, SSX2, Protein SSX2, Cancer/testis antigen 5.2, Tumor antigen HOM-MEL-40, CT5.2

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human colon adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human endometrium tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human bladder carcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human colon tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human ovary tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human ovary adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human breast adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (AB236415)

Paraffin-embedded human prostate tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (AB236415)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SSX2 stained for SSX2 (green) using ab236415 at 1/100 dilution in ICC/IF.

Western blot - Anti-SSX2 antibody [OTI4A11] (AB236415)
  • WB

Supplier Data

Western blot - Anti-SSX2 antibody [OTI4A11] (AB236415)

All lanes:

Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA at 5 µg

Predicted band size: 22 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4A11

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SSX2.

Q16385

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Clone OTI4A11 (formerly 4A11).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSX2 is a member of the synovial sarcoma X (SSX) breakpoint protein family. It performs mechanical function by acting as a transcriptional repressor. This protein also known as cancer/testis antigen 5.2 (CT5.2) has a mass of approximately 23 kDa. SSX2 expression is mostly restricted to testicular tissue in healthy adults but becomes anomalously expressed in various cancers making it a cancer/testis antigen of interest in research.
Biological function summary

SSX2 binds to other nuclear proteins suggesting it forms part of a regulatory complex affecting gene transcription. Such interactions influence cell cycle progression and other cellular processes. Given its involvement in transcriptional control SSX2 exerts significant influence over cellular proliferation and differentiation. Understanding the exact mechanisms by which SSX2 interacts with DNA and other proteins remains an area of keen scientific investigation because of its unique expression patterns.

Pathways

SSX2 impacts pathways related to transcriptional regulation and cellular growth. SSX2's involvement in the Wnt signaling pathway critical for cell proliferation illustrates its regulatory influence over cellular mechanisms. Furthermore its interaction with proteins like beta-catenin within these pathways can modulate various cellular outcomes affecting oncogenesis and tissue development.

SSX2 expression shows correlation with cancers such as melanoma and synovial sarcoma. The aberrant expression in these malignancies makes SSX2 a potential target for therapeutic interventions or a marker for diagnosis. Its link with MAGE proteins another family of cancer/testis antigens adds layers to its role in cancer biology. By better understanding SSX2's function and interactions researchers can advance therapeutic strategies to target its expression in cancerous cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could act as a modulator of transcription.
See full target information SSX2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2427415 PubMed39530604

2024

A positive feedback loop of SRSF9/USP22/ZEB1 promotes the progression of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Ming Hu,Jie Min,Xin Li
View all publications
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Product promise

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