AB199425

Anti-SSX2IP antibody [EPR16979]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal SSX2IP antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

KIAA0923, SSX2IP, Afadin- and alpha-actinin-binding protein, ADIP, Afadin DIL domain-interacting protein, SSX2-interacting protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (AB199425)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (AB199425)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (AB199425)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (AB199425)

SSX2IP was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199425 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab199425 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : Input HEK293 whole cell lysate (10 μg). Lane 2 : HEK293 whole cell lysate folowing precipitation. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199425 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST. 10 second exposure.

All lanes:

Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (ab199425)

Predicted band size: 71 kDa

Observed band size: 68 kDa,71 kDa

false

Supplier Data

Western blot - Anti-SSX2IP antibody [EPR16979] (AB199425)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/20000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 71 kDa

Observed band size: 68 kDa,71 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-SSX2IP antibody [EPR16979] (AB199425)

Based on the sequence analysis, ab199425 recognizes two isoforms with the predicted MWs of 71KDa, and 68KDa, respectively.

All lanes:

Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 71 kDa,77 kDa

Observed band size: 68 kDa,70 kDa,71 kDa

false

Exposure time: 1min

Carrier free

Anti-SSX2IP antibody [EPR16979] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16979

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/150", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SSX2IP also known as ADIP or SSX2-interacting protein is a protein weighing approximately 73 kDa. It functions mechanically as a centrosomal protein interacting with proteins involved in centrosome duplication and spindle formation. SSX2IP shows expression in various tissues especially in dividing cells highlighting its role in cell cycle regulation. It interacts with proteins like CCDC155 (CEP155) during centrosome dynamics.

Biological function summary

SSX2IP facilitates the assembly of the mitotic spindle and the proper segregation of chromosomes during cell division. It is part of a complex where it collaborates with spindle assembly factors to ensure accurate cell division. By supporting centrosome integrity SSX2IP plays a necessary role in maintaining genomic stability and proper cell proliferation.

Pathways

Several elements such as cell cycle progression and the SPICE1-interacting pathway rely on SSX2IP. It participates in pathways that oversee centrosome duplication and spindle dynamics making it critical for mitotic progression. SSX2IP interacts with proteins like SPDYA and PLK1 which are key elements in cell cycle-related pathways ensuring the fidelity of cell division.

Abnormalities in SSX2IP expression or function may have connections to cancer and neurodevelopmental disorders. In oncology dysregulation of SSX2IP can affect centrosome function and contribute to chromosomal instability a hallmark of many cancers. Additionally its interaction with abnormal centrosomal proteins like ASPM links it to microcephaly affecting neural development. Therefore understanding SSX2IP offers valuable insights into both cancer biology and developmental anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement : localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP : WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed : 23816619, PubMed : 26545777). Involved in ciliogenesis (PubMed : 24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed : 24356449).

See full target information SSX2IP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 49:188-200 PubMed39533770

2024

SSX2IP promotes cell proliferation and migration in breast cancer by regulating FANCI.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfu Liu,Xiaojing Zhang,Yansong Chen,Jingwei Tang,Hao Zhang,Gongsheng Jin

Toxicologic pathology 52:266-283 PubMed39193778

2024

Biocompatible Solutions: Evaluating the Safety of Repeated Intra-Articular Injections of pMPCylated Liposomes for Knee Osteoarthritis Therapy in Rat Models.

Applications

Unspecified application

Species

Unspecified reactive species

Yuval Ramot,Noam Kronfeld,Michal Steiner,Eric D Lee,Ronit Goldberg,Sabrina Jahn,Abraham Nyska

Turkish journal of haematology : official journal of Turkish Society of Haematology 39:160-169 PubMed35658330

2022

MiRNA-181b-5p Modulates Cell Proliferation, Cell Cycle, and Apoptosis by Targeting in Acute Lymphoblastic Leukemia

Applications

Unspecified application

Species

Unspecified reactive species

Xue Fu,Yuan He,Jun Song,Linhui Wang,Pengxiang Guo,Jun Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SSX2IP antibody [EPR16979]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (AB199425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (AB199425)

Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (AB199425)

Western blot - Anti-SSX2IP antibody [EPR16979] (AB199425)

Western blot - Anti-SSX2IP antibody [EPR16979] (AB199425)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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