Rabbit Recombinant Monoclonal SSX2IP antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (AB240348), expandable thumbnail](https://content.abcam.com/products/images/ssx2ip-antibody-epr16979-bsa-and-azide-free-ab240348--immunoprecipitation-img47295.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (AB240348)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (AB240348), expandable thumbnail](https://content.abcam.com/products/images/ssx2ip-antibody-epr16979-bsa-and-azide-free-ab240348--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88788.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (AB240348)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (AB240348), expandable thumbnail](https://content.abcam.com/products/images/ssx2ip-antibody-epr16979-bsa-and-azide-free-ab240348--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109607.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (AB240348)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed:23816619, PubMed:26545777). Involved in ciliogenesis (PubMed:24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed:24356449).
Alternative names
KIAA0923, SSX2IP, Afadin- and alpha-actinin-binding protein, ADIP, Afadin DIL domain-interacting protein, SSX2-interacting protein
Recommended products
Rabbit Recombinant Monoclonal SSX2IP antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16979
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240348 is the carrier-free version of Anti-SSX2IP antibody [EPR16979] ab199425.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SSX2IP also known as ADIP or SSX2-interacting protein is a protein weighing approximately 73 kDa. It functions mechanically as a centrosomal protein interacting with proteins involved in centrosome duplication and spindle formation. SSX2IP shows expression in various tissues especially in dividing cells highlighting its role in cell cycle regulation. It interacts with proteins like CCDC155 (CEP155) during centrosome dynamics.
- Biological function summary
SSX2IP facilitates the assembly of the mitotic spindle and the proper segregation of chromosomes during cell division. It is part of a complex where it collaborates with spindle assembly factors to ensure accurate cell division. By supporting centrosome integrity SSX2IP plays a necessary role in maintaining genomic stability and proper cell proliferation.
- Pathways
Several elements such as cell cycle progression and the SPICE1-interacting pathway rely on SSX2IP. It participates in pathways that oversee centrosome duplication and spindle dynamics making it critical for mitotic progression. SSX2IP interacts with proteins like SPDYA and PLK1 which are key elements in cell cycle-related pathways ensuring the fidelity of cell division.
- Associated diseases and disorders
Abnormalities in SSX2IP expression or function may have connections to cancer and neurodevelopmental disorders. In oncology dysregulation of SSX2IP can affect centrosome function and contribute to chromosomal instability a hallmark of many cancers. Additionally its interaction with abnormal centrosomal proteins like ASPM links it to microcephaly affecting neural development. Therefore understanding SSX2IP offers valuable insights into both cancer biology and developmental anomalies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348), expandable thumbnail](https://content.abcam.com/products/images/ssx2ip-antibody-epr16979-bsa-and-azide-free-ab240348--immunoprecipitation-img47295.jpg "Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348)")
Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348)
SSX2IP was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with Anti-SSX2IP antibody [EPR16979] ab199425 at 1/150 dilution. Western blot was performed from the immunoprecipitate using Anti-SSX2IP antibody [EPR16979] ab199425 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Input HEK293 whole cell lysate (10 µg). Lane 2: HEK293 whole cell lysate folowing precipitation. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SSX2IP antibody [EPR16979] ab199425 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SSX2IP antibody [EPR16979] ab199425).
All lanes: Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (Anti-SSX2IP antibody [EPR16979] ab199425)
Predicted band size: 71 kDa
Observed band size: 68 kDa, 71 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348), expandable thumbnail](https://content.abcam.com/products/images/ssx2ip-antibody-epr16979-bsa-and-azide-free-ab240348--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88788.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SSX2IP with Anti-SSX2IP antibody [EPR16979] ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SSX2IP antibody [EPR16979] ab199425).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348), expandable thumbnail](https://content.abcam.com/products/images/ssx2ip-antibody-epr16979-bsa-and-azide-free-ab240348--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109607.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] - BSA and Azide free (ab240348)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SSX2IP with Anti-SSX2IP antibody [EPR16979] ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SSX2IP antibody [EPR16979] ab199425).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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