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AB28266

Anti-ST14 antibody - Catalytic domain

5

(6 Reviews)

|

(5 Publications)

Rabbit Polyclonal ST14 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ST14.

View Alternative Names

PRSS14, SNC19, TADG15, ST14, Suppressor of tumorigenicity 14 protein, Matriptase, Membrane-type serine protease 1, Prostamin, Serine protease 14, Serine protease TADG-15, Tumor-associated differentially-expressed gene 15 protein, MT-SP1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody - Catalytic domain (AB28266)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody - Catalytic domain (AB28266)

ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ST14. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5Y6

Specificity

This antibody does not recognize matriptase 2 (MTSP2).

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Alternative Products

Primary Antibodies

AB235925

Anti-ST14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody (AB235925)

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Complementary Products

Assay Kits

AB314739

Human ST14 ELISA Kit

Sandwich ELISA - Human ST14 ELISA Kit (AB314739)

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Proteins & Peptides

AB114597

Recombinant Human ST14 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ST14 protein (GST tag N-Terminus) (AB114597)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB272721

Anti-HAI-1 antibody [EPR23294-49]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-HAI-1 antibody [EPR23294-49] (AB272721)

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Primary Antibodies

AB248225

Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST14 also known as matriptase is a serine protease with a molecular mass of about 92 kDa. It exists as a single-chain zymogen and activates through cleavage to form a two-chain form. Matriptase resides on the surface of epithelial cells and shows high expression in several tissues such as the epidermis prostate and gastrointestinal tract. This protease plays a part in tissue homeostasis by initiating proteolytic cascades that modify the extracellular matrix and influence cellular processes.
Biological function summary

Matriptase regulates proteolytic activation events in the extracellular environment. It instigates cascades that activate specific growth factors and proteases playing a role in cell proliferation and migration. Matriptase forms a complex with hepatocyte growth factor activator inhibitor-1 (HAI-1) which modulates its activity. The balance between matriptase and its inhibitors is important for controlling epithelial cell dynamics and maintaining a healthy cellular environment.

Pathways

Matriptase contributes to key signaling pathways like the epidermal growth factor receptor (EGFR) pathway and the plasminogen activation pathway. Within the EGFR signaling matriptase activates growth factors that influence cell proliferation and survival. It also intersects with proteins like epithelial growth factor receptor and plasminogen which are significant for cellular functions and homeostasis. These pathways are important for maintaining normal tissue function and responding to cellular stress.

Deregulation of matriptase is linked to conditions like cancer and ichthyosis. Matriptase overexpression or aberrant activity often relates to tumor progression and cancer metastasis particularly in breast and prostate cancer. Dysregulation also associates with the skin disorder ichthyosis due to imbalance with its inhibitor HAI-1 leading to defective epidermal permeability. Understanding matriptase's role in these diseases helps in designing therapeutic strategies and targeting the protease with specific inhibitors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (PubMed : 10373424). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (PubMed : 18843291). Proteolytically cleaves and therefore activates TMPRSS13 (PubMed : 28710277).
See full target information ST14
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed34623320

2021

T cell protein tyrosine phosphatase protects intestinal barrier function by restricting epithelial tight junction remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ronald R Marchelletta,Moorthy Krishnan,Marianne R Spalinger,Taylaur W Placone,Rocio Alvarez,Anica Sayoc-Becerra,Vinicius Canale,Ali Shawki,Young Su Park,Lucas Hp Bernts,Stephen Myers,Michel L Tremblay,Kim E Barrett,Evan Krystofiak,Bechara Kachar,Dermot Pb McGovern,Christopher R Weber,Elaine M Hanson,Lars Eckmann,Declan F McCole

Oncotarget 9:12682-12694 PubMed29560101

2018

Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pengming Sun,Lifang Xue,Yiyi Song,Xiaodan Mao,Lili Chen,Binhua Dong,Elena Loana Braicu,Jalid Sehouli

Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine 8:11-20 PubMed31131224

2018

THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER.

Applications

Unspecified application

Species

Unspecified reactive species

A V Kubyshkin,I I Fomochkina,A M Petrosyan

Molecular medicine reports 14:1465-74 PubMed27356668

2016

Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer.

Applications

WB

Species

Human

Pengming Sun,Zhongqing Jiang,Xiaofang Chen,Lifang Xue,Xiaodan Mao,Guanyu Ruan,Yiyi Song,Alexander Mustea

The British journal of dermatology 172:1407-11 PubMed25308318

2015

Whole-exome sequencing diagnosis of two autosomal recessive disorders in one family.

Applications

Unspecified application

Species

Unspecified reactive species

T Takeichi,A Nanda,S Aristodemou,J R McMillan,J Lee,M Akiyama,H Al-Ajmi,M A Simpson,J A McGrath
View all publications
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Product promise

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