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Rabbit Recombinant Monoclonal ST14 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600), expandable thumbnail
  • Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWBIPFlow Cyt
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (PubMed:10373424). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (PubMed:18843291). Proteolytically cleaves and therefore activates TMPRSS13 (PubMed:28710277).

Alternative names

PRSS14, SNC19, TADG15, ST14, Suppressor of tumorigenicity 14 protein, Matriptase, Membrane-type serine protease 1, Prostamin, Serine protease 14, Serine protease TADG-15, Tumor-associated differentially-expressed gene 15 protein, MT-SP1

Recommended products

Rabbit Recombinant Monoclonal ST14 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26785-94
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ST14 also known as matriptase is a serine protease with a molecular mass of about 92 kDa. It exists as a single-chain zymogen and activates through cleavage to form a two-chain form. Matriptase resides on the surface of epithelial cells and shows high expression in several tissues such as the epidermis prostate and gastrointestinal tract. This protease plays a part in tissue homeostasis by initiating proteolytic cascades that modify the extracellular matrix and influence cellular processes.

Biological function summary

Matriptase regulates proteolytic activation events in the extracellular environment. It instigates cascades that activate specific growth factors and proteases playing a role in cell proliferation and migration. Matriptase forms a complex with hepatocyte growth factor activator inhibitor-1 (HAI-1) which modulates its activity. The balance between matriptase and its inhibitors is important for controlling epithelial cell dynamics and maintaining a healthy cellular environment.

Pathways

Matriptase contributes to key signaling pathways like the epidermal growth factor receptor (EGFR) pathway and the plasminogen activation pathway. Within the EGFR signaling matriptase activates growth factors that influence cell proliferation and survival. It also intersects with proteins like epithelial growth factor receptor and plasminogen which are significant for cellular functions and homeostasis. These pathways are important for maintaining normal tissue function and responding to cellular stress.

Associated diseases and disorders

Deregulation of matriptase is linked to conditions like cancer and ichthyosis. Matriptase overexpression or aberrant activity often relates to tumor progression and cancer metastasis particularly in breast and prostate cancer. Dysregulation also associates with the skin disorder ichthyosis due to imbalance with its inhibitor HAI-1 leading to defective epidermal permeability. Understanding matriptase's role in these diseases helps in designing therapeutic strategies and targeting the protease with specific inhibitors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The blot was developed using a high sensitivity ECL substrate.

    The identity of the lower MW bands beneath 80 kDa are expected to be degraded ST14.

    Exposure times:

    Lanes 1: 59 seconds

    Lane 2 : 70 seconds

    All lanes: Western blot - Anti-ST14 antibody [EPR26785-94] (Anti-ST14 antibody [EPR26785-94] ab307599) at 1/1000 dilution

    Lane 1: Rat kidney tissue lysate at 20 µg

    Lane 2: NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa, 95 kDa

  • Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MDA-MB-231(PMID:28915606).

    The lane 5 was developed using a high sensitivity ECL substrate.
    The identity of the lower MW bands beneath 80 kDa are expected to be degraded ST14.

    Exposure times:

    Lanes 1, 3 and 4: 180 seconds

    Lane 2 : 70 seconds

    Lane 5 : 180 seconds

    All lanes: Western blot - Anti-ST14 antibody [EPR26785-94] (Anti-ST14 antibody [EPR26785-94] ab307599) at 1/1000 dilution

    Lane 1: NAMALWA (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg

    Lane 2: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 40 µg

    Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 4: HaCaT (human skin keratinocyte) whole cell lysate at 40 µg

    Lane 5: Mouse kidney tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa, 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ST14 with Anti-ST14 antibody [EPR26785-94] ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Negative control: no staining on human testis (PMID:12871983).
    The section was incubated with Anti-ST14 antibody [EPR26785-94] ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded cell pellets labeling ST14 with Anti-ST14 antibody [EPR26785-94] ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on (A) T-47D cell pellet, no staining on (B) MDA-MB-231 cell pellet.
    The section was incubated with Anti-ST14 antibody [EPR26785-94] ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ST14 with Anti-ST14 antibody [EPR26785-94] ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on rat colon.
    The section was incubated with Anti-ST14 antibody [EPR26785-94] ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ST14 with Anti-ST14 antibody [EPR26785-94] ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on mouse colon.
    The section was incubated with Anti-ST14 antibody [EPR26785-94] ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ST14 with Anti-ST14 antibody [EPR26785-94] ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human colon (PMID: 22081509).
    The section was incubated with Anti-ST14 antibody [EPR26785-94] ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (ab307600)

    This data was developed using Anti-ST14 antibody [EPR26785-94] ab307599, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling ST14 with Anti-ST14 antibody [EPR26785-94] ab307599 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
    Confocal image showing membranous staining in T-47D cell line, no staining was observed in MDA-MB-231 cell line.
    Negative control: MDA-MB-231(PMID:28915606).
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Product protocols

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