Anti-ST14 antibody [EPR26785-94] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ST14 with ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human testis (PMID : 12871983). The section was incubated with ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ST14 with ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID : 22081509). The section was incubated with ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded cell pellets labeling ST14 with ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) T-47D cell pellet, no staining on (B) MDA-MB-231 cell pellet. The section was incubated with ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling ST14 with ab307599 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in T-47D cell line, no staining was observed in MDA-MB-231 cell line. Negative control : MDA-MB-231(PMID : 28915606). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ST14 with ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon. The section was incubated with ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ST14 with ab307599 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon. The section was incubated with ab307599 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : MDA-MB-231(PMID : 28915606). The lane 5 was developed using a high sensitivity ECL substrate. The identity of the lower MW bands beneath 80 kDa are expected to be degraded ST14. Exposure times : Lanes 1, 3 and 4 : 180 seconds Lane 2 : 70 seconds Lane 5 : 180 seconds

All lanes:

Western blot - Anti-ST14 antibody [EPR26785-94] (<a href='/en-us/products/primary-antibodies/st14-antibody-epr26785-94-ab307599'>ab307599</a>) at 1/1000 dilution

Lane 1:

NAMALWA (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 40 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

HaCaT (human skin keratinocyte) whole cell lysate at 40 µg

Lane 5:

Mouse kidney tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,95 kDa

true

• WB

Supplier Data

Western blot - Anti-ST14 antibody [EPR26785-94] - BSA and Azide free (AB307600)

This data was developed using ab307599, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The blot was developed using a high sensitivity ECL substrate. The identity of the lower MW bands beneath 80 kDa are expected to be degraded ST14. Exposure times : Lanes 1 : 59 seconds Lane 2 : 70 seconds

All lanes:

Western blot - Anti-ST14 antibody [EPR26785-94] (<a href='/en-us/products/primary-antibodies/st14-antibody-epr26785-94-ab307599'>ab307599</a>) at 1/1000 dilution

Lane 1:

Rat kidney tissue lysate at 20 µg

Lane 2:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa,95 kDa

true