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AB228543

Anti-ST2 antibody

5

(9 Reviews)

|

(6 Publications)

Rabbit Polyclonal ST2 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IL1RL1.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

2 Images
Western blot - Anti-ST2 antibody (AB228543)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB228543)

10% SDS-PAGE

All lanes:

Western blot - Anti-ST2 antibody (ab228543) at 1/500 dilution

All lanes:

Rat colon tissue extract at 50 µg

Predicted band size: 63 kDa

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Western blot - Anti-ST2 antibody (AB228543)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB228543)

10% SDS-PAGE

All lanes:

Western blot - Anti-ST2 antibody (ab228543) at 1/500 dilution

All lanes:

RAW 264.7 (house macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Predicted band size: 124 kDa,48 kDa,63 kDa

Observed band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human IL1RL1. The exact immunogen used to generate this antibody is proprietary information.

Q01638

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Anti-Iba1 antibody [EPR16588] - Microglia marker

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 39:623-632 PubMed37916940

2023

mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianguo Song,Juan Dai,Xueping Chen,Fei Ding,Yanbo Ding,Liang Ma,Liwen Zhang

Frontiers in pharmacology 12:752734 PubMed34970140

2021

The Attenuating Effect of Low-Intensity Pulsed Ultrasound on Hypoxia-Induced Rat Chondrocyte Damage in TMJ Osteoarthritis Based on TMT Labeling Quantitative Proteomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Sa Du,Chao Liang,Yujie Sun,Bowen Ma,Wenmo Gao,Wei Geng

Cell cycle (Georgetown, Tex.) 18:3393-3403 PubMed31674275

2019

LncRNA ANRIL knockdown relieves myocardial cell apoptosis in acute myocardial infarction by regulating IL-33/ST2.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Yang,Xianwei Huang,Fudong Hu,Xin Fu,Zhengming Jiang,Kui Chen

The journal of pain 21:506-512 PubMed31562993

2019

Nociceptor Interleukin 33 Receptor/ST2 Signaling in Vibration-Induced Muscle Pain in the Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Alvarez,Oliver Bogen,Jon D Levine

International immunopharmacology 73:23-40 PubMed31078923

2019

Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Zhang,Jing Yang,Na Ao,Shi Jin,Ruiqun Qi,Fengping Shan,Jian Du

Cellular & molecular biology letters 23:52 PubMed30410547

2018

Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yafeng Liang,Nengli Yang,Guoquan Pan,Bingxin Jin,Shufen Wang,Wei Ji
View all publications
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Product promise

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