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AB233433

Anti-ST2 antibody

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(3 Publications)

Rabbit Polyclonal ST2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IL1RL1 aa 150-350.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-ST2 antibody (AB233433)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ST2 antibody (AB233433)

A549 (human lung carcinoma cell line) cells stained for ST2 using ab233433 at 20 μg/mL in ICC/IF. FITC-labeled secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody (AB233433)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody (AB233433)

Formalin-fixed, paraffin-embedded human kidney tissue stained for ST2 using ab233433 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-ST2 antibody (AB233433)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB233433)

All lanes:

Western blot - Anti-ST2 antibody (ab233433) at 2 µg/mL

All lanes:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 63 kDa

false

Western blot - Anti-ST2 antibody (AB233433)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB233433)

All lanes:

Western blot - Anti-ST2 antibody (ab233433) at 2 µg/mL

All lanes:

Human lung lysate

Predicted band size: 63 kDa

false

Western blot - Anti-ST2 antibody (AB233433)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB233433)

All lanes:

Western blot - Anti-ST2 antibody (ab233433) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Predicted band size: 63 kDa

false

Western blot - Anti-ST2 antibody (AB233433)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB233433)

All lanes:

Western blot - Anti-ST2 antibody (ab233433) at 2 µg/mL

All lanes:

Recombinant human ST2 protein

Predicted band size: 63 kDa

false

Western blot - Anti-ST2 antibody (AB233433)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody (AB233433)

All lanes:

Western blot - Anti-ST2 antibody (ab233433) at 2 µg/mL

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human IL1RL1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q01638

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine 264:9-19 PubMed38839357

2024

Shenqi Qiangjing Granules Ameliorate Asthenozoospermia in Mice by Regulating Ferroptosis through the METTL3/GPX4 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyu Lu,Jiabao Ma,Luying Wei,Jing Fu,Xiaoxia Li,Kedao Lai,Xin Li,Bingyu Xia,Bin Bin,Aicun Tang

Molecular medicine reports 23: PubMed33760194

2021

IL‑33/ST2 promotes the malignant progression of gastric cancer via the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Huang,Xing Cui,Wen Li,Chunlai Zhang,Liqing Liu,Jinxing Li

International journal of nanomedicine 15:10539-10546 PubMed33408473

2020

Development of a Novel Homogeneous Nanoparticle-Based Assay for Rapid and High-Throughput Quantitation of the sST2 Protein in Human Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Shenxia Gao,Junpu Li
View all publications
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