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AB288543

Anti-ST2 antibody [B-L42] - BSA and Azide free

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Mouse Monoclonal ST2 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL1RL1 aa 1-350.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

2 Images
Flow Cytometry - Anti-ST2 antibody [B-L42] - BSA and Azide free (AB288543)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ST2 antibody [B-L42] - BSA and Azide free (AB288543)

Flow cytometry overlay histogram of LPS-activated human monocytes labelling ST2 with ab288543 at 1/2 – 1/256 dilution (black) compared with a resting monocyte isotype control (grey). GAM-FITC at 1/800 dilution was used as the secondary antibody.

Western blot - Anti-ST2 antibody [B-L42] - BSA and Azide free (AB288543)
  • WB

Supplier Data

Western blot - Anti-ST2 antibody [B-L42] - BSA and Azide free (AB288543)

ST2 is described with 8 glycosylation sites which could increase the size of protein (Identification of human ST2 protein in the sera of patients with autoimmune diseases, Tominaga and col.)

Lanes 1 - 4:

Western blot - Anti-ST2 antibody [B-L42] - BSA and Azide free (ab288543) at 2 µg/mL

Lane 5:

Western blot - Anti-ST2 antibody [B-L42] - BSA and Azide free (ab288543)

Lane 1:

rh ST2 (non-reduced form)

Lane 2:

rh ST2 (reduced form)

Lane 3:

Cell lysate of HT29 (non-reduced form)

Lane 4:

Cell lysate of HT29 (reduced form)

Lane 5:

Molecular weight marker

Secondary

All lanes:

anti-IgG2b HRP at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-L42

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human IL1RL1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q01638

Specificity

Recognises both natural membrane form and recombinant human ST2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Hybridoma: Myeloma X63/AG.8653 x Balb/c node cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from cell culture supernatant
Storage buffer
pH: 7.3 - 7.5 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
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Product promise

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