AB25877

Anti-ST2 antibody - BSA free

Name: Anti-ST2 antibody - BSA free (ab25877) | Abcam
Brand: Abcam Limited
SKU: AB25877
Price: 480 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(33 Publications)

Anti-ST2 antibody - BSA free (ab25877) is a rabbit polyclonal antibody detecting ST2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- BSA free for higher signal-to noise ratio
- Over 20 publications
- Trusted since 2005

View Alternative Names

Ly84, St2, Ste2, Il1rl1, Interleukin-1 receptor-like 1, Interleukin-33 receptor alpha chain, Lymphocyte antigen 84, Protein ST2, Protein T1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

Human lung cancer tissue stained for ST2 using ab25877 at 5 μg/ml in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

Immunohistochemistry of ST2 in human liver tissue with ST2 antibody at 5 μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human lung cancer tissue labeling ST2 with Anti-ST2 antibody (ab25877) at 5μg/ml.

Immunohistochemistry (PFA fixed) - Anti-ST2 antibody - BSA free (AB25877)

IHC (PFA fixed)

Supplier Data

Immunohistochemistry (PFA fixed) - Anti-ST2 antibody - BSA free (AB25877)

Immunohistochemistry analysis of PFA-fixed human lung tissue labeling ST2 with ab25877 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence - Anti-ST2 antibody - BSA free (AB25877)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ST2 antibody - BSA free (AB25877)

Mouse kidney cells stained for ST2 using ab25877 at 20 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

ab25877 at 2μg/ml staining ST2 in mouse kidney tissue by IHC

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded rat kidney tissue labeling ST2 with ab25877 at 5 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supplier Data

Western blot - Anti-ST2 antibody - BSA free (AB25877)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-ST2 antibody - BSA free (ab25877) at 1 µg/mL

Lane 1:

293 cells in the absence of blocking peptide

Lane 2:

293 cells in the presence of blocking peptide

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC (PFA fixed), ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Il1rl1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P14719

Specificity

ab25877 reacts all three known ST2 isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCPFAfixed-species-checked": "testedAndGuaranteed", "IHCPFAfixed-species-dilution-info": "10 µg/mL", "IHCPFAfixed-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCPFAfixed-species-checked": "testedAndGuaranteed", "IHCPFAfixed-species-dilution-info": "10 µg/mL", "IHCPFAfixed-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "ab25877 at 2ug/ml. 1 hour incubation at room temperature in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "ab25877 at 2ug/ml. 1 hour incubation at room temperature in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ST2 antibody - BSA free (ab25877) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of ST2?
Anti-ST2 - BSA free (ab25877) specifically detects a band for ST2 (UniProt: P14719) at a molecular weight of 40kDa.

Trusted by the scientific community
Anti-ST2 - BSA free (ab25877) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ST2 Antibody is affinity chromatography purified via peptide column.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.

Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 17675517, PubMed : 18450470, PubMed : 22660580, PubMed : 29045903). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function (By similarity). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed : 34644537).. Isoform B. Inhibits IL-33 signaling.

See full target information Il1rl1

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:701 PubMed41053006

2025

Lactate-mediated histone lactylation promotes melanoma angiogenesis via IL-33/ST2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Zhao,Yuxuan Qian,Lin He,Taoxin Peng,Hanbin Wang,Xiangxu Wang,Linhan Jiang,Jinrong Fan,Hengxiang Zhang,Di Qu,Qing Zhu,Hao Wang,Shida Zhang,Chenyang Li,Xiwen Dong,Xianya Zhao,Huina Wang,Yuqi Yang,Xiuli Yi,Tao Zhao,Yu Liu,Jianglin Zhang,Guoqiang Zhang,Qiong Shi,Tianwen Gao,Chunying Li,Weinan Guo

Immunity, inflammation and disease 13:e70231 PubMed40849678

2025

Carbon Monoxide Alleviates Cardiomyocyte Pyroptosis in Diabetic Cardiomyopathy by Downregulating the IL-33/ST2L Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjie Jiang,Ping Zhu,Ping Yao,Xiaojun Bi,Chao Gao

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70085 PubMed40328686

2025

Decreased IL-33 Expression in the Cervix in LPS-Induced Preterm Birth and the Potential Role of Mast Cells: A Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sema Avci,Ciler Celik-Ozenci,Nilay Kuscu,Nayce Ilayda Bektas

Open life sciences 19:20220841 PubMed38585634

2024

IL-33/soluble ST2 axis is associated with radiation-induced cardiac injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokeya Yasen,Renaguli Aikebaier,Atiguli Maimaiti,Munire Mushajiang

Biomedicines 11: PubMed37893107

2023

Single-Cell RNA Sequencing (scRNA-seq) Identifies L1CAM as a Key Mediator between Epithelial Tuft Cell and Innate Lymphoid Cell in the Colon of Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guanying Bianca Xu,Yuan-Xiang Pan,Wenyan Mei,Hong Chen

Clinical, cosmetic and investigational dermatology 16:639-650 PubMed36936754

2023

IL-33 Contributes to the Pathological Changes of Hair Follicles in Psoriasis: A Potential Target for Psoriatic Alopecia.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Dai,Huoying Chen,Mengya Jiao,Na Zhang,Xuhuan Tang,Anqi Fan,Shiwang Liu,Zhigang Qian,Chenchen Wang,Yong Xu,Zheng Tan,Fanfan Zeng,Fang Zheng

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2202632 PubMed36373718

2022

Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Daishi Yamakawa,Junya Tsuboi,Kousuke Kasahara,Chise Matsuda,Yuhei Nishimura,Tatsuya Kodama,Naoyuki Katayama,Masatoshi Watanabe,Masaki Inagaki

Journal of molecular neuroscience : MN 72:730-740 PubMed34988900

2022

A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-inflammatory Activity and Improves Long-Term Functional Recovery in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qichao Wu,Wenkai Zhang,Shuo Yuan,Yanjun Zhang,Wenxiu Zhang,Yan Zhang,Xueming Chen,Lei Zang

The World Allergy Organization journal 14:100582 PubMed34659628

2021

Effect of parasympathetic inhibition on expression of ILC2 cells in a mouse model of allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Hu,Yan Wang,Guotong Zheng,Hailin Zhang,Liyan Ni

Cell reports 33:108553 PubMed33378665

2020

Airway-Associated Macrophages in Homeostasis and Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Anna E Engler,Alexandra B Ysasi,Riley M F Pihl,Carlos Villacorta-Martin,Hailey M Heston,Hanne M K Richardson,Bibek R Thapa,Noah R Moniz,Anna C Belkina,Sarah A Mazzilli,Jason R Rock

View all publications

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