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AB259749

Anti-ST2 antibody [EPR22369-79] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal ST2 antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

View Alternative Names

Fit1, Il1rl1, Interleukin-1 receptor-like 1, Fit-1, Fos-responsive gene 1 protein

1 Images
Sandwich ELISA - Anti-ST2 antibody [EPR22369-79] - BSA and Azide free (Capture) (AB259749)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ST2 antibody [EPR22369-79] - BSA and Azide free (Capture) (AB259749)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab255716).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22369-79

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab259749 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat ST2. The recommended pair for sandwich ELISA is:
Capture: ab259749, Rat ST2 (IL1RL1) Capture Antibody (unconjugated)
Detector: ab259750, Rat ST2 (IL1RL1) Detector Antibody (unconjugated)
The reference range value is 70.31 - 4500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (By similarity). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages.. Isoform B. Inhibits IL-33 signaling.
See full target information Il1rl1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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