Rabbit Monoclonal ST2 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071), expandable thumbnail](https://content.abcam.com/products/images/st2-antibody-epr25264-147-ab291071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400397.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071), expandable thumbnail](https://content.abcam.com/products/images/st2-antibody-epr25264-147-ab291071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400398.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071), expandable thumbnail](https://content.abcam.com/products/images/st2-antibody-epr25264-147-ab291071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400399.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Phytophthora infestans | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Phytophthora infestans, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Phytophthora infestans, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Phytophthora infestans, Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Phytophthora infestans | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Phytophthora infestans, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed:16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity). Isoform B. Inhibits IL-33 signaling.
Alternative names
DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2
Recommended products
Rabbit Monoclonal ST2 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25264-147
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
- Biological function summary
ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.
- Pathways
IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.
- Associated diseases and disorders
ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071), expandable thumbnail](https://content.abcam.com/products/images/st2-antibody-epr25264-147-ab291071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400397.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071)
Immunohistochemical analysis of paraffin-embedded human colon tissue labelling ST2 with ab29071 at 1/2000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on stromal cells of human colon is observed. The section was incubated with ab291071 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071), expandable thumbnail](https://content.abcam.com/products/images/st2-antibody-epr25264-147-ab291071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400398.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue labelling ST2 with ab29071 at 1/2000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on stromal cells of human endometrial cancer is observed. The section was incubated with ab291071 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071), expandable thumbnail](https://content.abcam.com/products/images/st2-antibody-epr25264-147-ab291071--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400399.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (ab291071)
Immunohistochemical analysis of paraffin-embedded A HEK-293T cells tr tissue labeling ST2 with ab291071 at 1/2000 (0.282 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Positive staining on HEK-293T cells transfected with a human IL1RL1 expression vector containing a myc-His tag (image A). No staining on HEK-293T cells untreated (image B). The section was incubated with ab291071 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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