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AB291071

Anti-ST2 antibody [EPR25264-147]

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Rabbit Recombinant Monoclonal ST2 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)

Immunohistochemical analysis of paraffin-embedded A HEK-293T cells tr tissue labeling ST2 with ab291071 at 1/2000 (0.282 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Positive staining on HEK-293T cells transfected with a human IL1RL1 expression vector containing a myc-His tag (image A). No staining on HEK-293T cells untreated (image B). The section was incubated with ab291071 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)

Immunohistochemical analysis of paraffin-embedded human colon tissue labelling ST2 with ab29071 at 1/2000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on stromal cells of human colon is observed. The section was incubated with ab291071 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody [EPR25264-147] (AB291071)

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue labelling ST2 with ab29071 at 1/2000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on stromal cells of human endometrial cancer is observed. The section was incubated with ab291071 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.

  • Carrier free

    Anti-ST2 antibody [EPR25264-147] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25264-147

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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