Rabbit Polyclonal ST3Gal I antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ST3GAL1 aa 1-200.
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Publications
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- Oncotarget 8:29013-290272017α2,3-sialyltransferase type I regulates migration and peritoneal dissemination of ovarian cancer cells.Applications:IHC, WBReactive species:Unspecified reactive speciesKuo-Chang Wen,Pi-Lin Sung,Shie-Liang Hsieh,Yu-Ting Chou,Oscar Kuang-Sheng Lee,Cheng-Wen Wu,Peng-Hui WangPubMed 28423672
- Laboratory investigation; a journal of technical m 96:731-402016Alpha-2, 3-sialyltransferases regulate the multidrug resistance of chronic myeloid leukemia through miR-4701-5p targeting ST3GAL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li et. al.PubMed 27088512
- BMC cancer 15:8802015Prognostic significance of ST3GAL-1 expression in patients with clear cell renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Bai,Li Liu,Yu Xia,Qilai Long,Jiajun Wang,Jiejie Xu,Jianming GuoPubMed 26552809
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ST3GAL1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q11201
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
A beta-galactoside alpha2-3 sialyltransferase involved in terminal sialylation of glycoproteins and glycolipids (PubMed:31784620, PubMed:8027041). Catalyzes the transfer of sialic acid (N-acetyl-neuraminic acid; Neu5Ac) from the nucleotide sugar donor CMP-Neu5Ac onto acceptor Galbeta-(1->3)-GalNAc-terminated glycoconjugates through an alpha2-3 linkage (PubMed:31784620, PubMed:8027041). Adds sialic acid to the core 1 O-glycan, Galbeta-(1->3)-GalNAc-O-Ser/Thr, which is a major structure of mucin-type O-glycans. As part of a homeostatic mechanism that regulates CD8-positive T cell numbers, sialylates core 1 O-glycans of T cell glycoproteins, SPN/CD43 and PTPRC/CD45. Prevents premature apoptosis of thymic CD8-positive T cells prior to peripheral emigration, whereas in the secondary lymphoid organs controls the survival of CD8-positive memory T cells generated following a successful immune response (By similarity). Transfers sialic acid to asialofetuin, presumably onto Galbeta-(1->3)-GalNAc-O-Ser (By similarity). Sialylates GM1a, GA1 and GD1b gangliosides to form GD1a, GM1b and GT1b, respectively (By similarity) (PubMed:8027041).
Alternative names
SIAT4, SIAT4A, ST3GAL1, Gal-NAc6S, Monosialoganglioside sialyltransferase, SIATFL, ST3Gal I, ST3GalA.1, ST3O, Sialyltransferase 4A, ST3GalI, SIAT4-A
Recommended products
Rabbit Polyclonal ST3Gal I antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ST3GAL1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ST3GAL1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q11201
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ST3Gal I also known as ST3 beta-galactoside alpha-23-sialyltransferase 1 or sialyltransferase 1 is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates specifically creating the sialyl T antigen. ST3Gal I is expressed in many tissues including the gastrointestinal tract lungs and the immune system reflecting its diverse roles in different physiological contexts.
- Biological function summary
ST3Gal I participates in glycosylation processes that modify proteins and lipids influencing cell-cell interactions immune responses and protein stability. It often associates with glycosylation complexes ensuring the proper addition of sialic acid to glycoconjugates. These modifications are critical for the formation of sialyated glycoconjugates which play significant roles in cellular communication and pathogen recognition.
- Pathways
ST3Gal I impacts the sialylation pathway and by extension affects glycan biosynthesis pathways. It modifies glycoproteins and glycolipids which are important in cell adhesion and signaling pathways. Within these pathways ST3Gal I shows interactions with proteins involved in these processes such as those in the selectin family which mediate leukocyte trafficking.
- Associated diseases and disorders
Altered ST3Gal I expression or activity links to certain cancers and immune disorders. Its dysregulation can lead to aberrant glycosylation patterns impacting processes like tumor metastasis and immune evasion. For instance the sialylation of mucins which influences tumor progression requires ST3Gal I. Also its interaction with other glycosyltransferases includes the regulation of immune cell surface antigens connecting it to autoimmune conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ST3Gal I antibody (ab96129)
12% SDS PAGE
All lanes: Western blot - Anti-ST3Gal I antibody (ab96129) at 1/500 dilution
All lanes: HeLa S3 whole cell lysate at 30 µg
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3Gal I antibody (ab96129)
ab96129 at 1/100 dilution staining ST3Gal I in paraffin-embedded serous OVCA by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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