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AB155022

Anti-ST3GAL3 antibody

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Rabbit Polyclonal ST3GAL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ST3GAL3 aa 100 to C-terminus.

View Alternative Names

SIAT6, ST3GAL3, ST3Gal III, ST3N, Sialyltransferase 6, ST3GalIII

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody (AB155022)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody (AB155022)

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor labeling ST3GAL3 with ab155022 at 1/250.

Western blot - Anti-ST3GAL3 antibody (AB155022)
  • WB

Unknown

Western blot - Anti-ST3GAL3 antibody (AB155022)

10% SDS PAGE

All lanes:

Western blot - Anti-ST3GAL3 antibody (ab155022) at 1/5000 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

IMR32 whole cell lysate at 30 µg

Predicted band size: 42 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody (AB155022)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody (AB155022)

Immunofluorescence analysis of methanol-fixed MCF7 labeling ST3GAL3 with ab155022 at 1/500 dilution. Lower image shows costaining with Hoechst 33342.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ST3GAL3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q11203

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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AB177959

Anti-ST3GAL3 antibody [EPR10006(2)]

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We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST3GAL3 also known as ST3 beta-galactoside alpha-23-sialyltransferase 3 is an enzyme involved in sialylation the process of adding sialic acid residues to glycoproteins and glycolipids. It has a molecular mass of approximately 42 kDa. ST3GAL3 primarily localizes in the Golgi apparatus where it catalyzes the transfer of sialic acid to galactose residues on glycoproteins. It is expressed in several tissues with notable levels in the brain and liver playing a role in glycan modification which affects cellular functions.
Biological function summary

ST3GAL3 contributes to the modification and stabilization of glycoproteins and glycolipids. It does not form part of a larger protein complex but interacts with substrates involved in glycan biosynthesis. These modifications can impact cell-cell interactions signaling and immune response. Its activity affects the surface properties of cells influencing processes like cell adhesion and migration which can be critical in development and differentiation.

Pathways

ST3GAL3 participates in glycosylation pathways that are essential for the proper functioning of the immune and nervous systems. It plays a role in the N-glycan biosynthesis pathway which is important for proper protein folding and trafficking. It also participates in the sialylation pathway alongside proteins such as ST3GAL4 and ST3GAL6 which serve similar functions but with different substrate specificities enabling a diverse range of glycan structures in cellular systems.

Mutations or dysregulation in ST3GAL3 has links to disorders like neurodevelopmental conditions including intellectual disability. Its altered expression can affect the function of proteins associated with cognitive development and neural connectivity. Changes in sialylation patterns can also relate to certain cancers where it influences tumor growth and metastasis. ST3GAL3 has interactions with other glycosyltransferases in these diseases which can exacerbate or ameliorate the pathological state. Understanding these interactions provides insights into potential therapeutic targets for treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.
See full target information ST3GAL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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