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Rabbit Recombinant Monoclonal ST3GAL3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (AB177959), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ST3GAL3 antibody [EPR10006(2)] (AB177959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody [EPR10006(2)] (AB177959), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (AB177959), expandable thumbnail
  • Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (AB177959), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted
Predicted
Rat
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ST3GAL3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10006(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ST3GAL3 also known as ST3 beta-galactoside alpha-23-sialyltransferase 3 is an enzyme involved in sialylation the process of adding sialic acid residues to glycoproteins and glycolipids. It has a molecular mass of approximately 42 kDa. ST3GAL3 primarily localizes in the Golgi apparatus where it catalyzes the transfer of sialic acid to galactose residues on glycoproteins. It is expressed in several tissues with notable levels in the brain and liver playing a role in glycan modification which affects cellular functions.

Biological function summary

ST3GAL3 contributes to the modification and stabilization of glycoproteins and glycolipids. It does not form part of a larger protein complex but interacts with substrates involved in glycan biosynthesis. These modifications can impact cell-cell interactions signaling and immune response. Its activity affects the surface properties of cells influencing processes like cell adhesion and migration which can be critical in development and differentiation.

Pathways

ST3GAL3 participates in glycosylation pathways that are essential for the proper functioning of the immune and nervous systems. It plays a role in the N-glycan biosynthesis pathway which is important for proper protein folding and trafficking. It also participates in the sialylation pathway alongside proteins such as ST3GAL4 and ST3GAL6 which serve similar functions but with different substrate specificities enabling a diverse range of glycan structures in cellular systems.

Associated diseases and disorders

Mutations or dysregulation in ST3GAL3 has links to disorders like neurodevelopmental conditions including intellectual disability. Its altered expression can affect the function of proteins associated with cognitive development and neural connectivity. Changes in sialylation patterns can also relate to certain cancers where it influences tumor growth and metastasis. ST3GAL3 has interactions with other glycosyltransferases in these diseases which can exacerbate or ameliorate the pathological state. Understanding these interactions provides insights into potential therapeutic targets for treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

    Immunohistochemical analysis of Human skeletal muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling ST3GAL3 using ab177959 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

    Immunofluorescence analysis of A673 cells labeling ST3GAL3 using ab177959 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

    Immunohistochemical analysis of fetal cardiac muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959), expandable thumbnail

    Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

    All lanes: Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959) at 1/1000 dilution

    Lane 1: Human fetal heart lysate at 10 µg

    Lane 2: Human skeletal muscle lysate at 10 µg

    Predicted band size: 42 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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