Rabbit Recombinant Monoclonal ST3GAL3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Tested | Tested |
Mouse | Predicted | Not recommended | Predicted | Predicted | Predicted |
Rat | Predicted | Not recommended | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Select an associated product type
Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.
SIAT6, ST3GAL3, ST3Gal III, ST3N, Sialyltransferase 6, ST3GalIII
Rabbit Recombinant Monoclonal ST3GAL3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
EPR10006(2)
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
ST3GAL3 also known as ST3 beta-galactoside alpha-23-sialyltransferase 3 is an enzyme involved in sialylation the process of adding sialic acid residues to glycoproteins and glycolipids. It has a molecular mass of approximately 42 kDa. ST3GAL3 primarily localizes in the Golgi apparatus where it catalyzes the transfer of sialic acid to galactose residues on glycoproteins. It is expressed in several tissues with notable levels in the brain and liver playing a role in glycan modification which affects cellular functions.
ST3GAL3 contributes to the modification and stabilization of glycoproteins and glycolipids. It does not form part of a larger protein complex but interacts with substrates involved in glycan biosynthesis. These modifications can impact cell-cell interactions signaling and immune response. Its activity affects the surface properties of cells influencing processes like cell adhesion and migration which can be critical in development and differentiation.
ST3GAL3 participates in glycosylation pathways that are essential for the proper functioning of the immune and nervous systems. It plays a role in the N-glycan biosynthesis pathway which is important for proper protein folding and trafficking. It also participates in the sialylation pathway alongside proteins such as ST3GAL4 and ST3GAL6 which serve similar functions but with different substrate specificities enabling a diverse range of glycan structures in cellular systems.
Mutations or dysregulation in ST3GAL3 has links to disorders like neurodevelopmental conditions including intellectual disability. Its altered expression can affect the function of proteins associated with cognitive development and neural connectivity. Changes in sialylation patterns can also relate to certain cancers where it influences tumor growth and metastasis. ST3GAL3 has interactions with other glycosyltransferases in these diseases which can exacerbate or ameliorate the pathological state. Understanding these interactions provides insights into potential therapeutic targets for treating these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of Human skeletal muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling ST3GAL3 using ab177959 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Immunofluorescence analysis of A673 cells labeling ST3GAL3 using ab177959 at 1/50 dilution.
Immunohistochemical analysis of fetal cardiac muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes: Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959) at 1/1000 dilution
Lane 1: Human fetal heart lysate at 10 µg
Lane 2: Human skeletal muscle lysate at 10 µg
Predicted band size: 42 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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