AB225793

Anti-ST6GAL1 antibody [EPR22054-238]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ST6GAL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SIAT1, ST6GAL1, ST6Gal I, Sialyltransferase 1, ST6GalI

4 Images

Flow Cytometry (Intracellular) - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HFF-1 (humanskin fibroblast cell line; Left panel) and Daudi (human Burkitt's lymphoma cell line; Right panel) cell lines labeling ST6GAL1 with ab225793 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : HFF-1 [PMID : 23358684].

Immunocytochemistry/ Immunofluorescence - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (human Burkitt's lymphoma cell line) and HFF-1 (human skin fibroblast cell line) cells labeling ST6GAL1 with ab225793 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining in Daudi cells, likely localized to the Golgi (PMID : 23578204).

Negative control : HFF-1 (human skin fibroblast) [PMID : 23358684].

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

ST6GAL1 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab225793 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225793 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Raji whole cell lysate 10 μg (Input).

Lane 2 : ab225793 IP in Raji whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab225793 in Raji whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-ST6GAL1 antibody [EPR22054-238] (ab225793)

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID 23358684).

Negative control : HFF-1 (PMID : 23358684).

All lanes:

Western blot - Anti-ST6GAL1 antibody [EPR22054-238] (ab225793) at 1/1000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 3:

HFF-1 (human skin fibroblast cell line) whole cell lysate at 10 µg

Lane 4:

THP-1 (human monocytic leukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

Carrier free

Anti-ST6GAL1 antibody [EPR22054-238] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22054-238

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST6GAL1 is an enzyme that belongs to the glycosyltransferase family specifically classified as a sialyltransferase. ST6GAL1 catalyzes the addition of sialic acid to glycoproteins and glycolipids impacting their function and stability. It has an approximate molecular mass of 45 kDa. ST6GAL1 is expressed in a variety of cell types including B lymphocytes and epithelial cells. Its expression can be observed on the cell surface where it plays important roles in cell-cell interactions.

Biological function summary

ST6GAL1 contributes to the mechanics of cell signaling and immune system modulation. ST6GAL1 is involved in the biosynthesis of sialylated glycoconjugates which affect cellular recognition and communication. This enzyme does not form part of a multi-protein complex but interacts with other proteins upon glycosylation events. Through its action ST6GAL1 impacts multiple cellular processes including inflammation regulation and pathogen recognition.

Pathways

ST6GAL1 plays roles in glycosylation and immune response mechanisms. It participates in the N-glycan biosynthesis pathway which influences protein folding and stability. Additionally ST6GAL1 impacts the sialylation pathway where it modifies the surface proteins for lymphocyte activation. Importantly ST6GAL1 interacts with proteins such as Siglecs which are involved in immune response modulation and cellular recognition.

ST6GAL1 is implicated in cancer progression and autoimmune diseases. In various cancers such as colon cancer altered ST6GAL1 expression affects metastatic potential and tumor cell survival. Additionally aberrant ST6GAL1 expression occurs in autoimmune diseases like lupus wherein it modulates immune cell activity. ST6GAL1 interacts with proteins like CD22 a sialic acid-binding lectin on B cells influencing disease pathogenesis and progression through immune system pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates. In B lymphocytes, generates neuraminidase-sensitive lymphocyte cell-surface differentiation antigens, such as CDw75, HB-6 and CD76 (PubMed : 1730763). Sialylates complex-type N-glycans attached on the fragment crystallizable (Fc) of IgGs confering anti-inflammatory effector functions. Preferentially monosialylates the alpha(1->3) mannose antenna of Fc glycoforms with subsequent disialylation occurring at a much slower rate.

See full target information ST6GAL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 60:3741-3757 PubMed36940077

2023

The Aldose Reductase Inhibitor Epalrestat Maintains Blood-Brain Barrier Integrity by Enhancing Endothelial Cell Function during Cerebral Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Tongshuai Zhang,Jinrong Wu,Xinmin Yao,Yao Zhang,Yue Wang,Yang Han,Yun Wu,Zhenyu Xu,Jing Lan,Siyu Han,Haifeng Zou,Qixu Sun,Dandan Wang,Jingyu Zhang,Guangyou Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ST6GAL1 antibody [EPR22054-238]

Flow Cytometry (Intracellular) - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Immunocytochemistry/ Immunofluorescence - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Immunoprecipitation - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Western blot - Anti-ST6GAL1 antibody [EPR22054-238] (AB225793)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise