AB275282

Anti-ST6GAL1 antibody [EPR22054-282] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ST6GAL1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.

View Alternative Names

SIAT1, ST6GAL1, B-cell antigen CD75, ST6Gal I, Sialyltransferase 1, ST6GalI

1 Images

Sandwich ELISA - Anti-ST6GAL1 antibody [EPR22054-282] - BSA and Azide free (Capture) (AB275282)

sELISA

Supplier Data

Sandwich ELISA - Anti-ST6GAL1 antibody [EPR22054-282] - BSA and Azide free (Capture) (AB275282)

Representative standard curve from corresponding SimpleStep ELISA^® Kit (ab275104).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22054-282

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab275282 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse ST6Gal1. The recommended pair for sandwich ELISA is:
Capture: ab275282, Mouse ST6Gal1 Capture Antibody (unconjugated)
Detector: ab259499, Mouse ST6Gal1 Detector Antibody (unconjugated)
The reference range value is 46.875 - 3000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST6GAL1 is an enzyme that belongs to the glycosyltransferase family specifically classified as a sialyltransferase. ST6GAL1 catalyzes the addition of sialic acid to glycoproteins and glycolipids impacting their function and stability. It has an approximate molecular mass of 45 kDa. ST6GAL1 is expressed in a variety of cell types including B lymphocytes and epithelial cells. Its expression can be observed on the cell surface where it plays important roles in cell-cell interactions.

Biological function summary

ST6GAL1 contributes to the mechanics of cell signaling and immune system modulation. ST6GAL1 is involved in the biosynthesis of sialylated glycoconjugates which affect cellular recognition and communication. This enzyme does not form part of a multi-protein complex but interacts with other proteins upon glycosylation events. Through its action ST6GAL1 impacts multiple cellular processes including inflammation regulation and pathogen recognition.

Pathways

ST6GAL1 plays roles in glycosylation and immune response mechanisms. It participates in the N-glycan biosynthesis pathway which influences protein folding and stability. Additionally ST6GAL1 impacts the sialylation pathway where it modifies the surface proteins for lymphocyte activation. Importantly ST6GAL1 interacts with proteins such as Siglecs which are involved in immune response modulation and cellular recognition.

ST6GAL1 is implicated in cancer progression and autoimmune diseases. In various cancers such as colon cancer altered ST6GAL1 expression affects metastatic potential and tumor cell survival. Additionally aberrant ST6GAL1 expression occurs in autoimmune diseases like lupus wherein it modulates immune cell activity. ST6GAL1 interacts with proteins like CD22 a sialic acid-binding lectin on B cells influencing disease pathogenesis and progression through immune system pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

See full target information ST6GAL1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ST6GAL1 antibody [EPR22054-282] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-ST6GAL1 antibody [EPR22054-282] - BSA and Azide free (Capture) (AB275282)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise