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AB69276

Anti-ST6GAL2 antibody

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(1 Publication)

Mouse Polyclonal ST6GAL2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ST6GAL2.

View Alternative Names

KIAA1877, SIAT2, ST6GAL2, ST6Gal II, Sialyltransferase 2, ST6GalII, hST6Gal II

2 Images
Western blot - Anti-ST6GAL2 antibody (AB69276)
  • WB

Unknown

Western blot - Anti-ST6GAL2 antibody (AB69276)

All lanes:

Western blot - Anti-ST6GAL2 antibody (ab69276) at 1/500 dilution

Lane 1:

ST6GAL2 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 60 kDa

Observed band size: 50 kDa

true

Western blot - Anti-ST6GAL2 antibody (AB69276)
  • WB

CiteAb

Western blot - Anti-ST6GAL2 antibody (AB69276)

ST6GAL2 western blot using anti-ST6GAL2 antibody ab69276. Publication image and figure legend from Liang, L., Xu, J., et al., 2018, Cell Death Dis, PubMed 29515098.

ab69276 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab69276 please see the product overview.

HCP5 functions as a ceRNA and sponges miR-22-3p, miR-186-5p and miR-216a-5p.a Using two programs (starbase and microRNA.org), we found that both HCP5 and ST6GAL2 are potential targets of miR-22-3p, miR-186-5p and miR-216a-5p. b, c A dual luciferase reporter assay was used to test whether HCP5 and ST6GAL2 are targets of miR-22-3p, miR-186-5p and miR-216a-5p. d Western blot analysis showed that the knockdown of miR-22-3p, miR-186-5p, or miR-216a-5p in FTC133 cells triggered a significant increase in ST6GAL2 protein expression. e, f An RIP experiment showed that HCP5 was enriched in Ago2-contaning miRNAs compared with IgG; miR-22-3p, miR-186-5p and miR-216a-5p were also detected in the same precipitate (*pvalue < 0.05)

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ST6GAL2.

Q96JF0

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST6GAL2 also known as ST6 beta-galactoside alpha-26-sialyltransferase 2 is an enzyme that catalyzes the transfer of sialic acid to galactose-containing substrates. This glycosylation process impacts the structure and function of glycoproteins and glycolipids. The mass of ST6GAL2 is approximately 56 kDa. It is mainly expressed in the brain and liver and also found in lower levels in some other tissues suggesting roles in specific physiological functions.
Biological function summary

The enzyme plays an integral role in the sialylation of glycan structures influencing cellular interactions and signaling pathways. It does not generally function as a component of a larger complex but works independently in modifying glycoproteins which are important for cell surface interactions neural development and immune response. The sialylation impacts the recognition and binding abilities of glycosylated molecules affecting both intra- and inter-cellular communication.

Pathways

This enzyme is vital in the sialylation branch of the glycosylation pathway which is important in the synthesis of complex carbohydrates on cell surfaces. This pathway intersects with insulin signaling where glycoproteins engage in regulatory feedback mechanisms. ST6GAL2 also relates to the immune response pathways where its activity modifies the surface antigens therefore impacting interactions with immune receptors.

Aberrant ST6GAL2 expression and activity link to neurodegenerative diseases like Alzheimer's and metabolic disorders such as diabetes. In Alzheimer's disease changes in glycoprotein sialylation can affect amyloid precursor protein processes whereas in diabetes altered sialylation impairs insulin receptor function. ST6GAL2 interacts with related proteins such as amyloid-beta precursor protein in neurodegeneration and insulin in diabetes influencing pathological progressions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.
See full target information ST6GAL2

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 9:372 PubMed29515098

2018

LncRNA HCP5 promotes follicular thyroid carcinoma progression via miRNAs sponge.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Liang,Jingchao Xu,Meng Wang,Gaoran Xu,Ning Zhang,Guangzhi Wang,Yongfu Zhao
View all publications

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