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AB229816

Anti-ST6GalNAc I antibody

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(2 Publications)

Rabbit Polyclonal ST6GalNAc I antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ST6GALNAC1 aa 1-250.

View Alternative Names

SIAT7A, UNQ543/PRO848, ST6GALNAC1, ST6GalNAc I, Sialyltransferase 7A, ST6GalNAc-I, ST6GalNAcI, hST6GalNAc-I, SIAT7-A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST6GalNAc I antibody (AB229816)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST6GalNAc I antibody (AB229816)

Paraffin-embedded human melanoma cancer tissue stained for ST6GalNAc I using ab229816 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST6GalNAc I antibody (AB229816)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST6GalNAc I antibody (AB229816)

Paraffin-embedded human skin tissue stained for ST6GalNAc I using ab229816 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ST6GalNAc I antibody (AB229816)
  • WB

Supplier Data

Western blot - Anti-ST6GalNAc I antibody (AB229816)

All lanes:

Western blot - Anti-ST6GalNAc I antibody (ab229816) at 1/500 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/50000 dilution

Predicted band size: 61 kDa,69 kDa

Observed band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ST6GALNAC1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NSC7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST6GalNAc I also known as ST6 N-acetylgalactosaminide alpha-26-sialyltransferase 1 plays an important mechanical role in glycosylation processes. It adds sialic acid to the terminal position of N-acetylgalactosamine on glycoproteins. The enzyme has a molecular weight of around 55 kDa. Expression of ST6GalNAc I is found predominantly in the Golgi apparatus where glycosylation occurs. It is expressed in tissues such as the liver heart and brain though expression levels can vary.
Biological function summary

ST6GalNAc I is involved in the regulation of cell-to-cell interactions and immune responses. It is not known to be a part of larger enzyme complexes but operates independently adding sialic acid residues that can modify the properties and functions of glycoproteins on the cell surface. These modifications can influence cell signaling migration and adhesion through their effects on the cell glycocalyx.

Pathways

ST6GalNAc I plays a role in the sialylation pathway which affects processes like cellular communication and protein stability. The enzyme's activity influences the O-glycan biosynthesis pathway. It works alongside other proteins such as ST3Gal I and ST6Gal I to fine-tune the sialylation states of glycoconjugates. This tuning can significantly impact interactions at the cell surface therefore influencing downstream signaling events.

ST6GalNAc I has a link to cancer and autoimmune disorders. Abnormal expression or activity of ST6GalNAc I can alter glycosylation patterns contributing to the progression of colon cancer by influencing tumor cell metastasis and immune cell evasion. Changes in its activity also have associations with autoimmune conditions where they may disrupt normal immune cell recognition and response. The target's modulation affects other proteins like MUC1 often implicated in cancer and immune-related anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein sialyltransferase specifically expressed in goblet cells that plays a key role in intestinal host-commensal homeostasis (PubMed : 35303419). Conjugates sialic acid with an alpha-2-6 linkage to N-acetylgalactosamine (GalNAc) glycan chains linked to serine or threonine in glycoproteins (PubMed : 16319059, PubMed : 35303419). Catalyzes the formation of the sialyl-Tn (S-Tn) antigen, an antigen found in intestinal goblet cells, as well as ulcerative colitis (UC) and various cancers (PubMed : 16319059, PubMed : 35303419). Protein sialylation in globlet cells is essential for mucus integrity and is required to protect the intestinal mucus against excessive bacterial proteolytic degradation (PubMed : 35303419).
See full target information ST6GALNAC1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40371640

2025

ST6GalNAc-I regulates tumor cell sialylation via NECTIN2/MUC5AC-mediated immunosuppression and angiogenesis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Muthamil Iniyan Appadurai,Sanjib Chaudhary,Ashu Shah,Gopalakrishnan Natarajan,Zahraa W Alsafwani,Parvez Khan,Dhananjay D Shinde,Subodh M Lele,Lynette M Smith,Mohd Wasim Nasser,Surinder Kumar Batra,Apar Kishor Ganti,Imayavaramban Lakshmanan

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:2546-2560 PubMed33818185

2021

A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse A Stokum,Bosung Shim,Weiliang Huang,Maureen Kane,Jesse A Smith,Volodymyr Gerzanich,J Marc Simard
View all publications
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