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AB69066

Anti-ST6GalNAc I antibody

5

(1 Review)

|

(1 Publication)

Mouse Polyclonal ST6GalNAc I antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ST6GALNAC1.

View Alternative Names

SIAT7A, UNQ543/PRO848, ST6GALNAC1, ST6GalNAc I, Sialyltransferase 7A, ST6GalNAc-I, ST6GalNAcI, hST6GalNAc-I, SIAT7-A

1 Images
Western blot - Anti-ST6GalNAc I antibody (AB69066)
  • WB

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Western blot - Anti-ST6GalNAc I antibody (AB69066)

All lanes:

Western blot - Anti-ST6GalNAc I antibody (ab69066) at 1/500 dilution

Lane 1:

ST6GalNAc I transfected 293T lysate at 25 µg

Lane 2:

Non-transfected 293T lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa,85 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ST6GALNAC1.

Q9NSC7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST6GalNAc I also known as ST6 N-acetylgalactosaminide alpha-26-sialyltransferase 1 plays an important mechanical role in glycosylation processes. It adds sialic acid to the terminal position of N-acetylgalactosamine on glycoproteins. The enzyme has a molecular weight of around 55 kDa. Expression of ST6GalNAc I is found predominantly in the Golgi apparatus where glycosylation occurs. It is expressed in tissues such as the liver heart and brain though expression levels can vary.
Biological function summary

ST6GalNAc I is involved in the regulation of cell-to-cell interactions and immune responses. It is not known to be a part of larger enzyme complexes but operates independently adding sialic acid residues that can modify the properties and functions of glycoproteins on the cell surface. These modifications can influence cell signaling migration and adhesion through their effects on the cell glycocalyx.

Pathways

ST6GalNAc I plays a role in the sialylation pathway which affects processes like cellular communication and protein stability. The enzyme's activity influences the O-glycan biosynthesis pathway. It works alongside other proteins such as ST3Gal I and ST6Gal I to fine-tune the sialylation states of glycoconjugates. This tuning can significantly impact interactions at the cell surface therefore influencing downstream signaling events.

ST6GalNAc I has a link to cancer and autoimmune disorders. Abnormal expression or activity of ST6GalNAc I can alter glycosylation patterns contributing to the progression of colon cancer by influencing tumor cell metastasis and immune cell evasion. Changes in its activity also have associations with autoimmune conditions where they may disrupt normal immune cell recognition and response. The target's modulation affects other proteins like MUC1 often implicated in cancer and immune-related anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein sialyltransferase specifically expressed in goblet cells that plays a key role in intestinal host-commensal homeostasis (PubMed : 35303419). Conjugates sialic acid with an alpha-2-6 linkage to N-acetylgalactosamine (GalNAc) glycan chains linked to serine or threonine in glycoproteins (PubMed : 16319059, PubMed : 35303419). Catalyzes the formation of the sialyl-Tn (S-Tn) antigen, an antigen found in intestinal goblet cells, as well as ulcerative colitis (UC) and various cancers (PubMed : 16319059, PubMed : 35303419). Protein sialylation in globlet cells is essential for mucus integrity and is required to protect the intestinal mucus against excessive bacterial proteolytic degradation (PubMed : 35303419).
See full target information ST6GALNAC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36293384

2022

Impact of Placental SLC2A3 Deficiency during the First-Half of Gestation.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron S Lynch,Victoria C Kennedy,Amelia R Tanner,Asghar Ali,Quinton A Winger,Paul J Rozance,Russell V Anthony
View all publications
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Product promise

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