JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB184777

Anti-ST8SIA5 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal ST8SIA5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ST8SIA5 aa 300 to C-terminus.

View Alternative Names

SIAT8E, ST8SIA5, Sialyltransferase 8E, Sialyltransferase St8Sia V, SIAT8-E, ST8SiaV

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ST8SIA5 antibody (AB184777)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ST8SIA5 antibody (AB184777)

Immunofluorescence analysis of RH-30 (human bone marrow metastasis cell line) whole cells labeling ST8SIA5 in the plasma membrane & midbody with ab184777 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (AB184777)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (AB184777)

Immunohistochemical analysis of human testis tissue labelled for ST8SIA5 using ab184777. Positive cytoplasmic staining observed in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (AB184777)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (AB184777)

Immunohistochemical analysis of human cerebral cortex tissue labelled for ST8SIA5 using ab184777. Positive cytoplasmic staining observed in neurons.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ST8SIA5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15466

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST8SIA5 also known as ST8 alpha-N-acetyl-neuraminide alpha-28-sialyltransferase 5 is an enzyme involved in sialylation a process that adds sialic acid to glycoproteins and glycolipids. This enzyme which has a molecular mass of approximately 45 kDa facilitates the transfer of sialic acid through alpha-28 glycosidic linkages. It shows expression in various tissues including the brain placenta and skeletal muscles. The expression levels of ST8SIA5 vary among tissues impacting its functional roles in those regions.
Biological function summary

ST8SIA5 influences cell-cell interactions modulation of immune responses and neural plasticity by altering cell surface molecules. It contributes to polysialylation integrating into glycan structures on cell membranes. Although it is not part of a larger complex it cooperates with other sialyltransferases to determine the complexity of sialic acid structures which affect cellular behavior.

Pathways

Sialylation by ST8SIA5 plays a significant role in the neural development and the regulation of immune responses. It interacts with pathways involving cell adhesion molecules influencing neuronal growth and migration. ST8SIA5 operates alongside proteins such as NCAM1 and ST8SIA2 impacting cell adhesion and signaling processes important in neural development and synaptic functions.

ST8SIA5 has associations with neurodevelopmental disorders and certain cancers. Alterations in its sialyltransferase activity may link to schizophrenia where it possibly interacts with proteins like NCAM1 to impact neural connectivity. Additionally variations in ST8SIA5 expression levels have been observed in tumor progression implicating a role in cellular transformation and metastasis potentially involving interactions with glycoproteins in cancer cells.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, GP1c and GT3 from GD1a, GT1b, GM1b and GD3 respectively.
See full target information ST8SIA5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Global medical genetics 10:388-394 PubMed38116489

2023

Integrative Analysis of to Identify a Novel Biomarker for Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxian Xiang,Li Wang,Yurong Cheng,Huanjuan An,Chan Zhang,Jing Wang,Yingying Tong,Dong Yan
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB126482

Anti-ZCWPW2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCWPW2 antibody (AB126482)

  • 0
  • 0
Primary Antibodies

AB184772

Anti-URG11 antibody

Immunocytochemistry/ Immunofluorescence - Anti-URG11 antibody (AB184772)

  • 4
  • 2
Primary Antibodies

AB188127

Anti-YIF1B antibody

Western blot - Anti-YIF1B antibody (AB188127)

  • 0
  • 0
Primary Antibodies

AB223477

Anti-ZKSCAN3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (AB223477)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com