Rabbit Polyclonal ST8SIA5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ST8SIA5 aa 300 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ST8SIA5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15466
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Target data
Function
Involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, GP1c and GT3 from GD1a, GT1b, GM1b and GD3 respectively.
Alternative names
SIAT8E, ST8SIA5, Sialyltransferase 8E, Sialyltransferase St8Sia V, SIAT8-E, ST8SiaV
Recommended products
Rabbit Polyclonal ST8SIA5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ST8SIA5 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ST8SIA5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15466
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ST8SIA5 also known as ST8 alpha-N-acetyl-neuraminide alpha-28-sialyltransferase 5 is an enzyme involved in sialylation a process that adds sialic acid to glycoproteins and glycolipids. This enzyme which has a molecular mass of approximately 45 kDa facilitates the transfer of sialic acid through alpha-28 glycosidic linkages. It shows expression in various tissues including the brain placenta and skeletal muscles. The expression levels of ST8SIA5 vary among tissues impacting its functional roles in those regions.
- Biological function summary
ST8SIA5 influences cell-cell interactions modulation of immune responses and neural plasticity by altering cell surface molecules. It contributes to polysialylation integrating into glycan structures on cell membranes. Although it is not part of a larger complex it cooperates with other sialyltransferases to determine the complexity of sialic acid structures which affect cellular behavior.
- Pathways
Sialylation by ST8SIA5 plays a significant role in the neural development and the regulation of immune responses. It interacts with pathways involving cell adhesion molecules influencing neuronal growth and migration. ST8SIA5 operates alongside proteins such as NCAM1 and ST8SIA2 impacting cell adhesion and signaling processes important in neural development and synaptic functions.
- Associated diseases and disorders
ST8SIA5 has associations with neurodevelopmental disorders and certain cancers. Alterations in its sialyltransferase activity may link to schizophrenia where it possibly interacts with proteins like NCAM1 to impact neural connectivity. Additionally variations in ST8SIA5 expression levels have been observed in tumor progression implicating a role in cellular transformation and metastasis potentially involving interactions with glycoproteins in cancer cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (ab184777)
Immunohistochemical analysis of human testis tissue labelled for ST8SIA5 using ab184777. Positive cytoplasmic staining observed in seminiferous ducts.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (ab184777)
Immunohistochemical analysis of human cerebral cortex tissue labelled for ST8SIA5 using ab184777. Positive cytoplasmic staining observed in neurons.
Immunocytochemistry/ Immunofluorescence - Anti-ST8SIA5 antibody (ab184777)
Immunofluorescence analysis of RH-30 (human bone marrow metastasis cell line) whole cells labeling ST8SIA5 in the plasma membrane & midbody with ab184777 at 2 μg/ml.
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