Anti-ST8SIA6 antibody [EPR29919-587]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse ST8SIA6 expression vector containing a his tag; (B) HEK-293T transfected with empty vector containing a his tag labeling ST8SIA6 with ab325041 at 1/100 (4.81 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse ST8SIA6 expression vector containing a his tag, and no staining in (B) HEK-293T transfected with empty vector containing a his tag.
The section was incubated with ab322460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ST8SIA6 with ab325041 at 1/100 (4.81 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Immunohistochemical analysis of paraffin-embedded Mouse squamous cell carcinoma tissue labeling ST8SIA6 with ab325041 at 1/100 (4.81 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse squamous cell carcinoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ST8SIA6 with ab325041 at 1/100 (4.81 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse kidney.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling ST8SIA6 with ab325041 at 1/100 (4.81 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse breast cancer.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Skeletal muscle, lung, liver, testis and brain (PMID : 11980897).

The highest expression of ST8SIA6 is observed in the kidney tissue and relatively low in the other tissues (PMID : 11980897).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ST8SIA6 antibody [EPR29919-587] (ab325041) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 60 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 60 µg

Lane 3:

Mouse lung tissue lysate at 60 µg

Lane 4:

Mouse liver tissue lysate at 60 µg

Lane 5:

Mouse testis tissue lysate at 60 µg

Lane 6:

Mouse brain tissue lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,80-90 kDa,36 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11980897; PMID : 8631818)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ST8SIA6 antibody [EPR29919-587] (ab325041) at 1/1000 dilution

Lane 1:

Mouse breast cancer tissue lysate at 40 µg

Lane 2:

Mouse skin cancer tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45-120 kDa,36 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-ST8SIA6 antibody [EPR29919-587] (AB325041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ST8SIA6 is a glycoprotein of approximately 45-120 kDa and is detected as a 35-80 kDa band after treatment with the deglycosylation enzyme Peptide : N-glycosidase F (PNGase F).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11980897; PMID : 8631818)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ST8SIA6 antibody [EPR29919-587] (ab325041) at 1/1000 dilution

Lane 1:

Untreated mouse breast cancer tissue lysate at 20 µg

Lane 2:

Mouse breast cancer tissue lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45-120 kDa,36 kDa

false

Exposure time: 48s