Rabbit Recombinant Monoclonal STAC antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ICC/IF | WB | Flow Cyt (Intra) | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Promotes expression of the ion channel CACNA1H at the cell membrane, and thereby contributes to the regulation of channel activity. Plays a minor and redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity. Slows down the inactivation rate of the calcium channel CACNA1C.
STAC1, STAC, STAC1, SH3 and cysteine-rich domain-containing protein, Src homology 3 and cysteine-rich domain-containing protein
Rabbit Recombinant Monoclonal STAC antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR12805
Affinity purification
Blue Ice
+4°C
+4°C
Do Not Freeze
ab250385 is the carrier-free version of Anti-STAC antibody [EPR12805] - N-terminal ab181157.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
STAC also known as SH3 and cysteine-rich domain 3 is a protein with a known molecular mass of approximately 38 kilodaltons. Structurally STAC contains a domain known as the SH3 domain which aids in protein-protein interactions. STAC expresses widely in various tissues including the brain heart and skeletal muscle. It interacts with other proteins through its cysteine-rich domain playing roles in cellular signaling.
Proteins with SH3 domains like STAC contribute to intracellular signaling pathways. STAC interacts within complexes that modulate ion channel activities which is important for cellular excitability. It holds an essential role in synaptic transmission and muscle contraction functions. The presence of STAC in the membrane and cytosolic compartments suggests its involvement in dynamic cellular processes.
STAC participates in both the calcium signaling pathway and the excitation-contraction coupling pathway. In calcium signaling it regulates calcium channels which are critical for maintaining cellular calcium homeostasis. Additionally STAC is involved with the excitation signal pathway by interacting with proteins such as RyR1 (Ryanodine Receptor 1) that facilitate calcium release in muscle contraction. This integration affects the overall physiological response during muscle excitation.
STAC shows an association with cardiac arrhythmias and muscular dystrophy. Mutations or altered expression in STAC can impact calcium homeostasis and muscle function contributing to these conditions. Additionally STAC links to the CACNA1S protein in muscular disorders associated with calcium channel dysfunction which further highlights its role in the pathophysiology of these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-STAC antibody [EPR12805] - N-terminal ab181157, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A431 cells (fixative 4% paraformaldehyde) labeling STAC with Anti-STAC antibody [EPR12805] - N-terminal ab181157 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution. Counter stained with Dapi (blue).
This data was developed using Anti-STAC antibody [EPR12805] - N-terminal ab181157, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of A431 cells labeling STAC using Anti-STAC antibody [EPR12805] - N-terminal ab181157 at a 1/20 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
This data was developed using Anti-STAC antibody [EPR12805] - N-terminal ab181157, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-STAC antibody [EPR12805] - N-terminal (Anti-STAC antibody [EPR12805] - N-terminal ab181157) at 1/2000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
This data was developed using Anti-STAC antibody [EPR12805] - N-terminal ab181157, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-STAC antibody [EPR12805] - N-terminal (Anti-STAC antibody [EPR12805] - N-terminal ab181157) at 1/10000 dilution
Lane 1: U87-MG lysate at 20 µg
Lane 2: 293 lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
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