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AB250385

Anti-STAC antibody [EPR12805] - BSA and Azide free

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Rabbit Recombinant Monoclonal STAC antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

STAC1, STAC, SH3 and cysteine-rich domain-containing protein, Src homology 3 and cysteine-rich domain-containing protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)

This data was developed using ab181157, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A431 cells (fixative 4% paraformaldehyde) labeling STAC with ab181157 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution. Counter stained with Dapi (blue).

Flow Cytometry (Intracellular) - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)

This data was developed using ab181157, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of A431 cells labeling STAC using ab181157 at a 1/20 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

Western blot - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)
  • WB

Supplier Data

Western blot - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)

This data was developed using ab181157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STAC antibody [EPR12805] - N-terminal (<a href='/en-us/products/primary-antibodies/stac-antibody-epr12805-n-terminal-ab181157'>ab181157</a>) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)
  • WB

Supplier Data

Western blot - Anti-STAC antibody [EPR12805] - BSA and Azide free (AB250385)

This data was developed using ab181157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STAC antibody [EPR12805] - N-terminal (<a href='/en-us/products/primary-antibodies/stac-antibody-epr12805-n-terminal-ab181157'>ab181157</a>) at 1/10000 dilution

Lane 1:

U87-MG lysate at 20 µg

Lane 2:

293 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12805

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250385 is the carrier-free version of ab181157.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAC also known as SH3 and cysteine-rich domain 3 is a protein with a known molecular mass of approximately 38 kilodaltons. Structurally STAC contains a domain known as the SH3 domain which aids in protein-protein interactions. STAC expresses widely in various tissues including the brain heart and skeletal muscle. It interacts with other proteins through its cysteine-rich domain playing roles in cellular signaling.
Biological function summary

Proteins with SH3 domains like STAC contribute to intracellular signaling pathways. STAC interacts within complexes that modulate ion channel activities which is important for cellular excitability. It holds an essential role in synaptic transmission and muscle contraction functions. The presence of STAC in the membrane and cytosolic compartments suggests its involvement in dynamic cellular processes.

Pathways

STAC participates in both the calcium signaling pathway and the excitation-contraction coupling pathway. In calcium signaling it regulates calcium channels which are critical for maintaining cellular calcium homeostasis. Additionally STAC is involved with the excitation signal pathway by interacting with proteins such as RyR1 (Ryanodine Receptor 1) that facilitate calcium release in muscle contraction. This integration affects the overall physiological response during muscle excitation.

STAC shows an association with cardiac arrhythmias and muscular dystrophy. Mutations or altered expression in STAC can impact calcium homeostasis and muscle function contributing to these conditions. Additionally STAC links to the CACNA1S protein in muscular disorders associated with calcium channel dysfunction which further highlights its role in the pathophysiology of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes expression of the ion channel CACNA1H at the cell membrane, and thereby contributes to the regulation of channel activity. Plays a minor and redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity. Slows down the inactivation rate of the calcium channel CACNA1C.
See full target information STAC
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com