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AB67773

Anti-STAC3 antibody

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Mouse Polyclonal STAC3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STAC3.

View Alternative Names

SH3 and cysteine-rich domain-containing protein 3, STAC3

1 Images
Western blot - Anti-STAC3 antibody (AB67773)
  • WB

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Western blot - Anti-STAC3 antibody (AB67773)

All lanes:

Western blot - Anti-STAC3 antibody (ab67773) at 1/500 dilution

Lane 1:

STAC3 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 42 kDa

Observed band size: 52 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human STAC3.

Q96MF2

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAC3 also known as SH3 and cysteine-rich domain 3 is a protein with a mass of approximately 35 kDa. It performs an important mechanical role in the process of excitation-contraction coupling in skeletal muscle cells. STAC3 is expressed mainly in skeletal muscle tissues where it joins other essential proteins to ensure proper muscle function. By interacting with other key proteins in the muscle STAC3 assists in the regulation of calcium ion release which is vital for muscle contraction and performance.
Biological function summary

STAC3 functions as part of a protein complex that includes the dihydropyridine receptor (DHPR) and the ryanodine receptor (RYR1) in skeletal muscles. It serves as a linking molecule within this complex facilitating communication between these receptors. This linkage is significant for maintaining the efficiency of calcium release during muscle activation. Without proper communication aided by STAC3 the calcium signaling in muscle cells becomes impaired which can affect muscle contraction.

Pathways

STAC3 plays an important role in the ECC (excitation-contraction coupling) pathway. This pathway is critical in converting an electrical signal to a mechanical response in muscle cells. It interacts specifically with DHPR and RYR1 which are central components in this signaling process. The interaction of STAC3 with these proteins helps in coordinating the sequence of events that leads to muscle contraction and relaxation showing how STAC3 integrates into these fundamental cellular processes.

Defects in STAC3 link to congenital myopathies specifically Native American myopathy (NAM). This disorder severely affects muscle function leading to symptoms like muscle weakness and skeletal deformities. Through its relation to NAM STAC3 also connects with mutations in RYR1 which is another protein implicated in muscle disorders. Understanding STAC3's role offers potential for developing therapeutic strategies targeting this protein to alleviate muscle-associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for normal excitation-contraction coupling in skeletal muscle and for normal muscle contraction in response to membrane depolarization. Required for normal Ca(2+) release from the sarcplasmic reticulum, which ultimately leads to muscle contraction. Probably functions via its effects on muscle calcium channels (PubMed : 23736855, PubMed : 29078335). Increases CACNA1S channel activity, in addition to its role in enhancing the expression of CACNA1S at the cell membrane. Has a redundant role in promoting the expression of the calcium channel CACNA1S at the cell membrane (By similarity). Slows down the inactivation rate of the calcium channel CACNA1C (PubMed : 29078335).
See full target information STAC3
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