Anti-STAM antibody [RP23040090]

• WB

Supplier Data

Western blot - Anti-STAM antibody [RP23040090] (AB308114)

Western blot analysis was performed on whole cell extracts of Caco-2 (Lane 1), A431 (Lane 2), A-375 (Lane 3), A549 (Lane 4), Caki-1 (Lane 5) and DU 145 (Lane 6). The blots were probed with ab308114, 2µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjµgate (0.4µg/mL, 1 : 2500 dilution). A ~ 60 kDa band corresponding to STAM was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-STAM antibody [RP23040090] (ab308114) at 2 µg/mL

Lane 1:

Caco-2 whole cell extract

Lane 2:

A431 whole cell extract

Lane 3:

A-375 whole cell extract

Lane 4:

A549 whole cell extract

Lane 5:

Caki-1 whole cell extract

Lane 6:

DU-145 whole cell extract

Secondary

All lanes:

Goat anti Rabbit IgG (H+L) Secondary Antibody, HRP conjµgate at 1/2500 dilution

Predicted band size: 58 kDa

Observed band size: 60 kDa

true

• WB

Supplier Data

Western blot - Anti-STAM antibody [RP23040090] (AB308114)

Western blot analysis of STAM in whole cell extracts from mouse brain using ab308114 at a dilution of 2.5 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~58kDa.

All lanes:

Western blot - Anti-STAM antibody [RP23040090] (ab308114) at 2.5 µg/mL

All lanes:

Mouse brain whole cell extracts

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit secondary antibody

Observed band size: 58 kDa

true