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Rabbit Recombinant Multiclonal STAM1 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human STAM.

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Images

Western blot - Anti-STAM antibody [RP23040090] (AB308114), expandable thumbnail
  • Western blot - Anti-STAM antibody [RP23040090] (AB308114), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Synthetic Peptide within Human STAM. Database link Q92783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species

Human

Dilution info

2-2.5 µg/mL

Notes

-

Species

Mouse

Dilution info

2.5 µg/mL

Notes

-

Target data

Function

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.

Alternative names

Signal transducing adapter molecule 1, STAM-1, STAM1, STAM

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Rabbit Recombinant Multiclonal STAM1 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human STAM.

Alternative names

Signal transducing adapter molecule 1, STAM-1, STAM1, STAM

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Synthetic Peptide within Human STAM. Database link Q92783
Clone number

RP23040090

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-STAM antibody [RP23040090] (ab308114), expandable thumbnail

    Western blot - Anti-STAM antibody [RP23040090] (ab308114)

    Western blot analysis of STAM in whole cell extracts from mouse brain using ab308114 at a dilution of 2.5 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~58kDa.

    All lanes: Western blot - Anti-STAM antibody [RP23040090] (ab308114) at 2.5 µg/mL

    All lanes: Mouse brain whole cell extracts

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit secondary antibody

    Developed using the ECL technique.

    Observed band size: 58 kDa

  • Western blot - Anti-STAM antibody [RP23040090] (ab308114), expandable thumbnail

    Western blot - Anti-STAM antibody [RP23040090] (ab308114)

    Western blot analysis was performed on whole cell extracts of Caco-2 (Lane 1), A431 (Lane 2), A-375 (Lane 3), A549 (Lane 4), Caki-1 (Lane 5) and DU 145 (Lane 6). The blots were probed with ab308114, 2µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjµgate (0.4µg/mL, 1:2500 dilution). A ~ 60 kDa band corresponding to STAM was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-STAM antibody [RP23040090] (ab308114) at 2 µg/mL

    Lane 1: Caco-2 whole cell extract

    Lane 2: A431 whole cell extract

    Lane 3: A-375 whole cell extract

    Lane 4: A549 whole cell extract

    Lane 5: Caki-1 whole cell extract

    Lane 6: DU-145 whole cell extract

    Secondary

    All lanes: Goat anti Rabbit IgG (H+L) Secondary Antibody, HRP conjµgate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa

    Observed band size: 60 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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