JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155527

Anti-STAM1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal STAM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STAM aa 100-400.

View Alternative Names

STAM1, STAM, Signal transducing adapter molecule 1, STAM-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB155527)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB155527)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling STAM with ab155527 at 1/500 dilution.

Western blot - Anti-STAM1 antibody (AB155527)
  • WB

Unknown

Western blot - Anti-STAM1 antibody (AB155527)

10% SDS PAGE

All lanes:

Western blot - Anti-STAM1 antibody (ab155527) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Lane 3:

K562 whole cell lysate at 30 µg

Lane 4:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Western blot - Anti-STAM1 antibody (AB155527)
  • WB

Supplier Data

Western blot - Anti-STAM1 antibody (AB155527)

All lanes:

Western blot - Anti-STAM1 antibody (ab155527) at 1/1000 dilution

All lanes:

C2C12 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human STAM aa 100-400. The exact immunogen used to generate this antibody is proprietary information.

Q92783

style
left-column

Alternative Products

Primary Antibodies

AB244470

Anti-STAM1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-STAM1 antibody (AB244470)

  • 0
  • 0
Primary Antibodies

AB117813

Anti-STAM1 antibody

Immunoprecipitation - Anti-STAM1 antibody (AB117813)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32043

Anti-STAT5a antibody [E289]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (AB32043)

  • 5
  • 2
Primary Antibodies

AB155539

Anti-HGS antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (AB155539)

  • 4
  • 3
Primary Antibodies

AB36569

Anti-Reptin/TIP49B/RUVB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

  • 4
  • 13
Primary Antibodies

AB179503

Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAM1 also known as Signal Transducing Adaptor Molecule 1 functions mechanically as an important player in intracellular signal transduction. It operates as an adaptor protein that interacts with various molecules within the cell. STAM1 has a molecular mass of approximately 58 kDa. Researchers observe high expression of STAM1 in the cytoplasmic region of cells being particularly abundant in immune cells. It engages in various protein-protein interactions to exert its function.
Biological function summary

STAM1 participates in endocytic trafficking and cytokine receptor endocytosis. It often associates with the ESCRT-0 (Endosomal Sorting Complex Required for Transport) complex which plays an essential role in sorting ubiquitinated cargo for lysosomal degradation. This protein helps regulate the cellular response to certain cytokines by facilitating the downregulation of activated receptors thereby contributing to signal attenuation.

Pathways

STAM1 involvement can be seen in cytokine signaling pathways and the ubiquitin-proteasome system. In the context of cytokine signaling STAM1 interacts with components like the Janus Kinase (JAK) family and Signal Transducers and Activators of Transcription (STATs) modulating cell growth and immune responses. It also has a significant function in the ubiquitin-dependent endocytic route coordinating with other ESCRT components like HRS (hepatocyte growth factor-regulated tyrosine kinase substrate).

Research links STAM1 with oncogenesis and neurodegenerative conditions. In cancer aberrant STAM1 expression correlates with altered growth factor receptor degradation potentially affecting tumor progression. STAM1's interaction with key proteins like c-MET a receptor tyrosine kinase can influence oncogenic signaling. In neurodegenerative disorders such as Alzheimer's disease STAM1's role in protein trafficking and degradation may contribute to the accumulation of misfolded proteins emphasizing its importance in maintaining cellular homeostasis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.. (Microbial infection) Plays an important role in Dengue virus entry.
See full target information STAM
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed39404738

2024

Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Yee Tey,Almut Dufner,Klaus-Peter Knobeloch,Jonathan N Pruneda,Michael J Clague,Sylvie Urbé

Cell death discovery 8:426 PubMed36284090

2022

Ferrostatin-1 alleviates tissue and cell damage in diabetic retinopathy by improving the antioxidant capacity of the Xc-GPX4 system.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhi Shao,Zhouxian Bai,Lirong Zhang,Fengyan Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com