AB244470

Anti-STAM1 antibody

Name: Anti-STAM1 antibody (ab244470) | Abcam
Brand: Abcam Limited
SKU: AB244470
Price: 630 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal STAM1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STAM aa 100-200.

View Alternative Names

STAM1, STAM, Signal transducing adapter molecule 1, STAM-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-STAM1 antibody (AB244470)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAM1 antibody (AB244470)

PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for STAM1 (green) using ab244470 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB244470)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB244470)

Paraffin-embedded human bone marrow tissue stained for STAM1 using ab244470 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-STAM1 antibody (AB244470)

All lanes:

Western blot - Anti-STAM1 antibody (ab244470) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 59 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human STAM aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q92783

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAM1 also known as Signal Transducing Adaptor Molecule 1 functions mechanically as an important player in intracellular signal transduction. It operates as an adaptor protein that interacts with various molecules within the cell. STAM1 has a molecular mass of approximately 58 kDa. Researchers observe high expression of STAM1 in the cytoplasmic region of cells being particularly abundant in immune cells. It engages in various protein-protein interactions to exert its function.

Biological function summary

STAM1 participates in endocytic trafficking and cytokine receptor endocytosis. It often associates with the ESCRT-0 (Endosomal Sorting Complex Required for Transport) complex which plays an essential role in sorting ubiquitinated cargo for lysosomal degradation. This protein helps regulate the cellular response to certain cytokines by facilitating the downregulation of activated receptors thereby contributing to signal attenuation.

Pathways

STAM1 involvement can be seen in cytokine signaling pathways and the ubiquitin-proteasome system. In the context of cytokine signaling STAM1 interacts with components like the Janus Kinase (JAK) family and Signal Transducers and Activators of Transcription (STATs) modulating cell growth and immune responses. It also has a significant function in the ubiquitin-dependent endocytic route coordinating with other ESCRT components like HRS (hepatocyte growth factor-regulated tyrosine kinase substrate).

Research links STAM1 with oncogenesis and neurodegenerative conditions. In cancer aberrant STAM1 expression correlates with altered growth factor receptor degradation potentially affecting tumor progression. STAM1's interaction with key proteins like c-MET a receptor tyrosine kinase can influence oncogenic signaling. In neurodegenerative disorders such as Alzheimer's disease STAM1's role in protein trafficking and degradation may contribute to the accumulation of misfolded proteins emphasizing its importance in maintaining cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.. (Microbial infection) Plays an important role in Dengue virus entry.

See full target information STAM

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 40:108 PubMed39630363

2024

The mechanism of sevoflurane affecting ovarian cancer cell proliferation and migration by regulating RNA methylase TRDMT1 to activate the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Huang,Xuewei Lao,Chengyan He,Jia Wang,Ying Pan

Frontiers in oncology 12:795971 PubMed35814368

2022

Expression Promotes Lung Adenocarcinoma Progression and Is Associated With Poor Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Jiang,Xiaolu Huang,Xiang Yang,Huixin Zhou,Yumin Wang

Frontiers in oncology 11:611081 PubMed33959493

2021

STAM Prolongs Clear Cell Renal Cell Carcinoma Patients' Survival Inhibiting Cell Growth and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Tuo Deng,Zihao He,Xiaolu Duan,Di Gu,Chao Cai,Wenqi Wu,Yongda Liu,Guohua Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAM1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-STAM1 antibody (AB244470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB244470)

Western blot - Anti-STAM1 antibody (AB244470)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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