Anti-STAM2 antibody [EPR8688] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-STAM2 antibody [EPR8688] - BSA and Azide free (AB248995)

This data was developed using ab151545, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling STAM2 with ab151545 at 1/100 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody [EPR8688] - BSA and Azide free (AB248995)

This data was developed using ab151545, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling STAM2 with ab151545 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-STAM2 antibody [EPR8688] - BSA and Azide free (AB248995)

This data was developed using ab151545, the same antibody clone in a different buffer formulation.

Overlay histogram showing HeLa cells stained with ab151545 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151545, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-STAM2 antibody [EPR8688] - BSA and Azide free (AB248995)

This data was developed using ab151545, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STAM2 antibody [EPR8688] (<a href='/en-us/products/primary-antibodies/stam2-antibody-epr8688-ab151545'>ab151545</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 58 kDa

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