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Rabbit Recombinant Monoclonal Stanniocalcin 2/STC-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.


Images

Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (AB255610), expandable thumbnail
  • Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (AB255610), expandable thumbnail
  • Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (AB255610), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (AB255610), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (AB255610), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.

Alternative names

Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2

Recommended products

Rabbit Recombinant Monoclonal Stanniocalcin 2/STC-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22866-10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.

Biological function summary

STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.

Pathways

In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.

Associated diseases and disorders

Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    STC2 was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate with ab255610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255610 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate 10ug

    Lane 2: ab255610 IP in T-47D whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab255610 in T-47D whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Predicted band size: 33 kDa

    Observed band size: 33-35 kDa

  • Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID:15367391, 25463045, 26361149).

    Exposure time: Lane 1: 3 minutes; Lane 2: 48 seconds.

    All lanes: Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610) at 1/1000 dilution

    Lane 1: HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: Human breast cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature, the dual bands caused by different posttranslational modifications (PMID: 15367391, 25463045, 26361149).

    Exposure time: Lane 1-2: 48 seconds; Lane 3: 26 seconds; Lane 4: 114 seconds; Lane 5: 15 seconds.

    All lanes: Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Lanes 3 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33-35 kDa

  • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer (A) and its adjacent noncancerous breast tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong cytoplasmic staining in human breast cancer tissue (A) while weak staining in its adjacent noncancerous breast tissue (B) (PMID: 18492817) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous lung tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong cytoplasmic staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous lung tissue (B) (PMID: 25463045) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in T-47D cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab255610 anti-STC2 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

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